The images, taken by award-winning royal photographer Chris Jackson, capture efforts to make a difference to the lives of the vulnerable children of Lesotho.
The exhibition at the Getty Images Gallery in London will be open to the public from 26th July until 10th August.
Prince Harry said of the charity's work: "In the absence of parents for the vast majority of Lesotho children we at Sentebale we hope are filling the gap as best we can.
"The stigma that surrounds HIV and Aids must be explained and, I hope, understood", he added.
Since it was co-founded by Prince Harry and Prince Seeiso of Lesotho in 2006, Sentebale has been providing healthcare and education to those most in need.
The exhibition features a variety of images; from portraits of the harsh reality of daily life for many orphans and herd boys, to the positive outcomes of those children with disabilities receiving an education.
Cathy Ferrier, chief executive of Sentebale, said: "We have ambitious plans to expand, including building a specialist centre for children living with HIV which will allow us to reach many more young people in Lesotho.
"We would like to invite everyone along to the exhibition to see for themselves the difference we are making to the children."
The show will be open to the public with free admission and prints are available of all the images with a percentage of proceeds going to Sentebale.