Tottenham defender Danny Rose will not recover in time to be fit for Saturday's FA Cup semi-final against Chelsea.
Rose has begun training again after missing almost three months with a knee injury but the full-back will not make the clash at Wembley.
Erik Lamela and Harry Winks are both out with hip and ankle injuries respectively while goalkeeper Michel Vorm is also unavailable with a knee problem.
Provisional squad: Lloris, Lopez, Whiteman, Davies, Alderweireld, Vertonghen, Dier, Wimmer, Walker, Trippier, Wanyama, Dembele, Eriksen, Alli, Sissoko, Onomah, Son, Janssen, Kane.
Gary Cahill is a major doubt for Chelsea after being admitted to hospital this week, according to reports.
Several national newspapers have reported that Cahill was admitted to hospital on Tuesday after training and released on Thursday following treatment and tests on a 'mystery illness'.
Chelsea head coach Antonio Conte is scheduled to speak at a pre-match media conference at 1415 BST on Friday afternoon, where he will be questioned about Cahill's condition.
Cahill played in Chelsea's loss at Manchester United last Sunday, when the Premier League leaders' advantage over nearest rivals Spurs was cut to four points. There are six games remaining.
Goalkeeper Thibaut Courtois missed the United clash with an ankle injury and Marcos Alonso withdrew during the warm-up at Old Trafford due to a stomach bug, the Daily Telegraph said.
Victor Moses and Diego Costa played despite a similar bug, but Cahill's illness is unrelated, the Telegraph added.
Chelsea are seeking a win over Tottenham which, as well as securing a place in the FA Cup final, would boost morale and confidence for the Premier League run-in.