Nearly 150 people who are disabled or in need of urgent care were evacuated from hospital in Aleppo Old City overnight, the International Committee of the Red Cross (ICRC) said.
Eleven dead were also taken from the hospital.
Those evacuated in the joint operation between the ICRC and the Syrian Arab Red Crescent were said to be "trapped in the area for days" following heavy nearby clashes.
A doctor involved in the evacuation said there was "much suffering" and people have had "no access to food or basics for almost a week".
Russian Foreign Minister Sergei Lavrov is due to meet US Secretary of State John Kerry in Hamburg on Thursday after the two men held talks on Wednesday.
Yesterday the Kremlin said a potential US-Russia deal to allow Syrian rebels to safely leave Aleppo was still on the agenda.