Norfolk cyclist Jody Cundy has apologised for his expletive-ridden rant after he was refused a re-start in the 1km time trial.
The cyclist, a C4 rider, lost his balance as he attempted to push off from the start line.
Boos echoed around the arena as the crowd repeatedly chanted "let him ride" after the ruling was announced over the loudspeaker system.
His devastated family, who were watching trackside, questioned the decision which left his mum Ann in tears.
"Why, why did they do this to him? They should have let him ride. Why didn't they let him ride? They didn't want him to win. He's so angry now," she said.
The decision provoked an expletive-laden rant from Cundy who threw his water bottle in frustration.
Members of Cundy's family had disbelief written all over their faces.
His mother Ann was in tears as she was supported by his brother. His father meanwhile was led away by one of the Paralympic volunteers.
Cundy was angered and upset as he shouted and threw his arms in the air.
A group of family and friends were wearing white t-shirts with the words "Jody London 2012" on the back.
Originally from Wisbech in Cambridgeshire, Cundy said after the mis-start: "I fell out of the gate because the f****** gate didn't open. I've wasted four years of my life to ride in front of 6,000 people.
"Do you know what it's like to dedicate four years of your life? I can't even express it. There aren't the f****** words. I'll never, ever get this opportunity again. Never, ever. What am I supposed to do now?"
Despite his disappointment, Cundy will have the chance to make amends when he takes part in the team pursuit.