Cambridge completed a Wembley double this season as they defeated Gateshead 2-1 in the Skrill Premier play-off final to seal a return to the Football League after a nine-year absence.
Goals from Liam Hughes and Ryan Donaldson, against his old club, put the U's in control but veteran striker Jack Lester pulled one back to set up a tense final 10 minutes for Richard Money's men.
For Cambridge, it was a second victory in less than two months at the national stadium after they beat Gosport 4-0 in March to lift the FA Trophy.
Donaldson, who swapped Gateshead for Cambridge last summer, almost gave the U's an early lead but Adam Bartlett was not to be caught cold and tipped away his long-range shot for a corner.
James Marwood twice went close with efforts from outside the area before Jamie Chandler worked goalkeeper Adam Smith.
Smith, on loan from Premier League newcomers Leicester, had to be alert early in the second half to keep out another Marwood attempt.
Cambridge responded through substitute Harrison Dunk, whose shot was tipped wide by Bartlett, and they took the lead after 51 minutes when Donaldson's cross drifted over the keeper for Hughes to head home from two-yards out.
Bartlett pushed over another header from Hughes shortly afterwards as the U's sought a second goal.
It came in the 71st minute after Ben Clark brought down Donaldson, who got to his feet and curled home the resulting free-kick from 22 yards out. His third goal at Wembley this season having netted twice against Gosport.
Jack Lester pulled one back for Gateshead with less than ten minutes to go,
An injury to Cambridge defender Ian Miller saw the U's finish the game with 10 men but, despite the fact almost 10 minutes of stoppage time was added the U's held on to record an historic win.