Football League new boys Cambridge United have been handed a trip to a Championship side for their first game in the Capital One Cup in ten years.
The U's will host former 2011 cup winners Birmingham City, who avoided relegation from the SkyBet Championship on the last day of the season.
Cambridge celebrated promotion back to the Football League in May after winning the Skrill Promotion Final against Gateshead.
Skrill Conference Premier champions Luton Town - who won the competition in 1988 - were drawn at home to Swindon Town, former winners in 1969. The clubs have never met in this competition before but both share the distinction of beating Premiership giants Arsenal in the final.
The Hatters will resume life in the Football League for the first time in five years when the new season starts in August.
One of the most eye-catching ties sees League One MK Dons against AFC Wimbledon of League Two.
The clubs meet for only the second time since the Dons' formation following the demise of the old Wimbledon FC, following their FA Cup clash in December 2012. They'll face each other again at Stadium MK where the hosts won 2-1.
MK Dons were one of the seeded teams in the First Round Draw. The others - Ipswich Town and Peterborough United - have been drawn away. Ipswich will visit League One Crawley Town while Posh travel to League Two Portsmouth.
Northampton Town, who secured their Football League status on the last day of the season will face Wolves who went up to the Championship as League One Champions. It'll be their third meeting in four years with the Cobblers losing their previous two - both at Sixfields.
Meanwhile League Two Stevenage - who knocked out Ipswich in the First Round last season - have another chance to be giant killers against another Championship side. Boro' are paired at home to Watford.