There were more than 600 cases of abusive behaviour towards staff at the East of England Ambulance service last year.
Figures obtained by ITV News Anglia show challenging and aggressive behaviour cases more then doubled compared to the year before.
Verbal abuse cases also went up, with 374 incidents.
The Trust is now launching a 'Don't Choose to Abuse' campaign to highlight the problem and remind people that there is zero tolerance against any form of abuse.
The Trust has a zero tolerance approach to violence against staff and works closely with police to prosecute offenders.