Jeremy Corbyn has said he wishes Clive Lewis well and "looks forward to working with him in the future" after he resigned from the shadow cabinet.
Mr Corbyn has told Labour MPs to back the Brexit Bill at its third reading, something Mr Lewis said he could not do.
The Labour leader said: “I would like to thank Clive for his work in the shadow cabinet, which has underlined what an asset he is to the Labour Party and our movement.
“I understand the difficulties MPs representing constituencies which voted Remain have in relation to the European Union Withdrawal Bill. MPs have a duty to represent their constituents as well as their party.
“However, the Labour Party respects the outcome of the EU referendum, so we have asked all Labour MPs to vote for the Bill at its third reading tonight.
“We have been clear from the start that Labour will not frustrate the triggering of Article 50, which represents the start of the process for leaving the EU."
Clive Lewis posted this picture of Star Trek's Mr Spock.
It refers to the 'no win scenario' that cadets in the United Federation of Planets must go through before becoming officers.
Our Political Reporter Emma Hutchinson got some early reaction to Clive Lewis' decision to stand down.
In response to @labourlewis resignation Jeremy Corbyn says he wishes him well and looks forward to working with him in the future
Mr Lewis had pledged to vote against the bill if it went through unamended. The majority of his constituency voted to remain in the EU referendum.
Clive Lewis says "cannot vote for something I believe will ultimately harm the city I have the honour to represent, love and call home"
Norwich South MP and Shadow business secretary Clive Lewis and has resigned from the shadow cabinet in order to defy Labour leader Jeremy Corbyn's order to back the European Union (Notification of Withdrawal) Bill at third reading, its final Commons stage.
- Video report by ITV News Anglia's Russell Hookey
Measures aimed at fixing Britain's "broken housing market" have been unveiled by ministers today.
The proposals include the introduction of new "Lifetime ISAs" to help first-time buyers save for a deposit, and a fund to help small house-building firms.
We've been assessing the current situation in Cambridgeshire where there's been a decline of more than 30% in the number of houses being built in the last 25 years.
Our Political Correspondent Emma Hutchinson spoke with Daniel Zeichner about why he had decided to defy the whip and vote against the bill.Read the full story ›
A protest against new American president Donald Trump has been staged in Norwich tonight.
Around 1,000 people gathered outside City Hall to show their anger at the travel ban introduced by the president last week.
Demonstrators also made it clear they opposed Mr Trump's proposed state visit to the UK later this year.
The protest was organised by a number of groups in the city, including 'Stand Up To Racism'.
The Shadow Business Secretary has told ITV News Anglia he would "walk" from the shadow cabinet if the Article 50 Bill is not amended.Read the full story ›
There are growing concerns over the impact that building Sizewell C could have on one of the region's most beautiful areas of countryside.Read the full story ›