Luton Town's famous win makes headlines in the United States
Chelmsford will visit Crawley while Cambridge City and Braintree Town could host League sides in Round Two.
The Assistant Manager of MK Dons Mick Harford has praised Cambridge City, who drew against the Dons in the first round of the FA Cup.
Saturday's FA Cup 4th Round tie between Norwich City and Luton Town is a repeat of the 1959 cup semi-final, which Luton eventually won after a replay.
As part of the build up to the game Jim Rice caught up with Terry Allcock, a veteran of Norwich City's '59ers', to talk about the Canaries' cup run and the modern side.
Saturday's football fixtures in the Anglia regionFA CUP
Aston Villa v IPSWICH TOWN
LUTON v Wolves
Sheff Wed v MK Dons
SOUTHEND v Brentford
Crewe v STEVENAGE
Doncaster Rovers v COLCHESTER UNITED
NORTHAMPTON TOWN v Fleetwood
All matches kick off at 3pm
Luton Town defender Ronnie Henry hopes his own personal experience of being an FA Cup giant killer will count for a lot when they face Championship strugglers Wolves on Saturday. Henry featured in Stevenage's big cup shocks in the past two seasons.
Boro knocked out Newcastle United and Reading, and held Tottenham to a draw before losing a 5th Round replay last March.
Henry, who joined Luton after being released by Stevenage last summer, said: 'They were great occasions for me and great occasions for the club. I'm looking forward to Saturday equally.
It'll be a great game at Kenilworth Road. I should imagine it's sold out already, and the atmosphere will be great'. Henry acknowledges an FA Cup 3rd round tie of such magnitude will be a new experience for some of Luton's BlueSquare Bet Premier Division players.
'I will just tell them to enjoy it', said Henry. 'The FA Cup is very special. It's one of the best trophies in the world in my opinion.
'Things can happen to you in the FA Cup. People can get moves, if you have a good game you never know what can happen to you. The occasions are very special so they have to enjoy it because they don't come around too often'.
The two clubs are separated by 60 places, but it's not that long ago when both Luton and Wolves were playing in the same division. Their last meeting was six seasons ago in the Championship when Wolves won 3-2 at Kenilworth Road.
However Luton will fancy their chances of causing a cup sensation with Wolves on a horrible run of four defeats in five games.
'It's a big game between two big Clubs, albeit at different ends of the league spectrum', Henry added.
'At the moment Wolves are in a bit of bother but they have some tremendous players and they're a great side. They'll be hard to beat'.
Four of our teams face FA Cup replays this evening. MK Dons will meet AFC Wimbledon in round Two if they beat Cambridge City. Northampton are away at Bradford, while Luton travel to Nuneaton.
Click on fixture for live updates on MK Dons v Cambridge City.
Click on fixture for live updates on Bradford City v Northampton Town.
Click on fixture for live updates on Nuneaton v Luton Town click on the link below.
It's a big night for Braintree Town in the FA Cup. The Conference side from Essex host the highest ranked team in the competition at this stage - League One leaders Tranmere.
Tranmere are clear leaders in their division - and sit 66 places above Braintree in the English football ladder.
The original game had to be called off due to the weather, but the pitch is fine tonight, and the game will go ahead.
Watch the game live on ITV4 (kick-off 7:45pm) or click here.
It was a weekend of contrasts for our Essex non-league clubs. Following Braintree's disappointment, there was elation for Chelmsford City who beat neighbours Colchester United from League One.
It was the first competitive match between the sides in 44 years - and one Clarets fans will remember for a long time to come.
Click here for more information.
Football minnows Arlesey Town from Bedfordshire will play a former FA Cup winners Coventry City in the first round of the competition. Russell Hookey has been finding out more about the team and the town.