The Toys ‘R’ Us store in Cambridge is one of 26 scheduled to close in Spring 2018.
It comes just months after the US-based retailer filed for bankruptcy.
In total it is understood that 500 to 800 jobs could be lost nationwide. In total at the moment they have 84 stores in the UK and employs a total of 3,200 people.
Steve Knights, managing director of Toys R Us UK, said the warehouse-style stores opened by the retailer in the 1980s and 1990s have proven "too big and expensive to run", adding that "newer, smaller, more interactive stores in the right shopping locations" were trading well.