The Dumfriesshire based Halo Trust is the latest charity to face questions about the conduct of a member of staff.
The charity, which has its global headquarters in Thornhill, clears land mines around the world.
It says a member of staff in Myanmar has been suspended following an allegation of sexual assault, which he denies.
A land mine charity in Dumfries, once supported by Princess Diana, has suspended its founder and chief executive.
The Halo Trust said there had been a "serious deterioration" in relations between Guy Willoughby and the board, and it had decided to suspend him.
An anti-landmine charity, based in south west Scotland, says it is thrilled at the boost it has received from Prince Harry on his visit to the United States.
The Prince has opened the HALO Trust's new exhibition, in Washington DC.
The charity was supported by the Princess of Wales; her youngest son is patron of its 25th anniversary.
Kim Inglis reports:
For more information on the HALO Trust and to find out how you can help, visit their website here.
Prince Harry received a welcome reserved for pop stars when his tour of America began - and a glowing tribute from first lady Michelle Obama.
Hundreds of screaming women greeted him on Washington's Capitol Hill when he attended an event promoting the Halo Trust, an anti-landmine charity he supports.
Prince Harry recalled the passion of his mother Princess Diana when he gave a speech in America and spoke of 'people who live in countries blighted by land mines' for who there could be 'no return to normality':
And when he was announced as a surprise guest at a White House tea reception honouring military mothers he received a standing ovation and more whoops of delight from US servicewomen and their children.
The seven-day visit is the Prince's first to America since he caused outrage by being photographed naked during a weekend break in Las Vegas last year
Prince Harry's support of a landmine clearance charity was praised today as he began his tour of the US by visiting an exhibition highlighting the organisation's vital work.
Harry made the trip to Capitol Hill in Washington to see a moving photographic display which is raising awareness about the efforts of the anti-landmine charity the Halo Trust.
Fiona Willoughby, wife of Guy Willoughby Halo's CEO said, "Having Prince Harry attend our exhibition makes it a global issue".
"People have forgotten about it and we think Prince Harry following in his mother's footsteps is a worthy cause and will raise the profile of what we are doing", she added.