Stranraer put an end to Rangers 100% winning record this season as they held the Ibrox giants to a 1-1 draw.
Rangers captain Lee McCulloch scored a first-half penalty to put them ahead, but Jamie Longworth hit back with an injury time equaliser.
It is a big night for Queen of the South as they play Rangers at Palmerston Park in the quarter final of the Ramsdens Cup.
Queen of the South are the Cup holders and they beat Rangers on the way to the final last season, in a dramatic penalty shootout.
Extra security is in place to ensure it is a good night on and off the pitch for the 7000 fans.
Kim Inglis reports:
Police Scotland are advising fans travelling to tonight's Queen of the South game against Rangers to travel early.
"High numbers of supporters are expected to attend the match so there will be increased levels of traffic and pedestrians in the area.
"If going to the game travel early to avoid the congestion and please note that no parking cones will be in place, so park in areas further from the ground.
"Officers will be policing the event to ensure everyone’s safety and we ask all fans behave sensibly and consider others, including local residents, before, during and after the match. We hope everyone enjoys the big game."
Queen of the South welcome Rangers to Palmerston Park tonight for the quarter-final of the Ramsdens Cup.
The Doonhamers knocked the Glasgow giants out of the cup at the same stage last year after a dramatic penalty shoot-out at Ibrox.
Jim McIntyre's side are hoping to get back to winning ways after their home defeat to Raith Rovers, but Ally McCoist's men will be coming to Dumfries looking for revenge.
More than 6,000 tickets have already been sold, but there are still some seats left for Queen of the South fans.
Tickets for the game, which kicks off at 7.45pm, can be bought at Palmerston Park until 3pm today.
Rangers boss Ally McCoist has confirmed he is interested in Queen of the South striker Nicky ClarkRead the full story ›
Rangers manager Ally McCoist has confirmed his interest in Queen of the South striker Nicky Clark.
The Light Blues boss has already held talks with the player's representative and his father, former Gers player and current Palmerston assistant Sandy, about a switch to Ibrox next season.
McCoist now hopes to meet with the player, who has netted an impressive 37 goals for the Irn-Bru Second Division champions this season, in the coming days
I've spoken to Nicky's agent and I've spoken to his father, who I know reasonably well, just to let them know that we have an interest in talking to Nicky and meeting up with him and seeing if there is a possibility of him coming to the club.
"We are no further down the line than that, but I spoke to Sandy and I'm very keen to meet up, as are Sandy and Nicky, so hopefully we can get a meeting in the next couple of days.
"His goalscoring record is fantastic and we certainly need strengthened in forward areas, as we do all over the park.
Annan Athletic were beaten 3-0 by Rangers in front of 42,000 fans at Ibrox.
They travelled to Glasgow to face the league leaders in Division Three.
The Gers managed to put one past Annan when Templeton scored within the first half hour.
The home side were then reduced to 10 men, after Lee Wallace was shown a red card for bringing down Jack Steele in the box.
Despite their one man advantage, Annan conceded another two goals in the second half, giving Rangers a six point lead at the top of the table.
Goals: Templeton ('29 and '70) and Little ('64)
Sent Off : Wallace ('59)
Ref : Paul Robertson
Annan Athletic held Glasgow Rangers to a goalless draw in the biggest game in the club's history. A sell-out crowd packed into the Galabank Stadium for the first time ever for the tie.
The first half of Annan's historic game has ended in a goalless draw. The club are playing Glasgow Rangers and it's the first time there has ever been a sell-out crowd at Galabank Stadium.