Scottish Natural Heritage have released a statement to ITV Border describing the risk of crayfish transfer to silage bales as "very small."
"It is the first time that we have come across reports of signal crayfish being transported in silage bales.
"Although it's feasible that crayfish might get caught up in the bales the risks of transfer of live crayfish between river catchments is very small."
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Early enquiries suggest the men may have taken the drug Blue Valium. Two of them remain in hospital with breathing problems.
More than 8,000 people are expected to attend over the three day event, which starts at 7pm tonight.
The local MP and leader of the Liberal Democrats says it was a "joyful" day for locals and former locals alike.