The World Owl Trust say their current agreement with the estate managers is being terminated and unless they can negotiate new arrangements, they'll have to leave by May 2015.
The charity claims the new terms are not financially viable, but the estate say negotiations are ongoing and in tough financial times it's having to review the way the owl centre is run.
The Muncaster estate says it's willing to negotiate but the Trust says it's looking for new premises. Honorary President Tony Warbuton said: "the owl trust is looking for somewhere new to be based, we do not feel we are wanted anymore at Muncaster."
More top news
A look ahead at the forecast for the Border region.
Around 4,000 people gathered at Whitehaven Rugby League Ground to question health officials about the West Cumberland Hospital.
A look at the forecast for the Border region