The World Owl Trust say their current agreement with the estate managers is being terminated and unless they can negotiate new arrangements, they'll have to leave by May 2015.
The charity claims the new terms are not financially viable, but the estate say negotiations are ongoing and in tough financial times it's having to review the way the owl centre is run.
The Muncaster estate says it's willing to negotiate but the Trust says it's looking for new premises. Honorary President Tony Warbuton said: "the owl trust is looking for somewhere new to be based, we do not feel we are wanted anymore at Muncaster."
More top news
A look ahead at the Bank Holiday forecast for the Border region.
Cumbria County Council will start work on a £320,000 resurfacing scheme on 6 June, between 6.00pm and 12.30am.
ITV Border's favourite Tapir has left the Lake District Wildlife Park near Keswick, to travel to his new home in the United Arab Emirates.