Staff from the Yorkshire Ambulance Service belonging to the Unite union have announced they will strike this weekend.
The strike will take place across the county from 3pm on Saturday to 7am on Sunday morning.
The union has three hundred and seventy five members in the service.
The crux of the dispute is the introduction of longer shift patterns that could mean staff working ten hours without a meal break.
The union has said that it is only a matter of time before someone is seriously injured or killed as a result of staff exhaustion.
In a statement the Trust said it has "taken all reasonable procedural steps to resolve the dispute and has fully cooperated with the course of action agreed with the Advisory, Conciliation and Arbitration Service (ACAS)."