Roy Hodgson has warned Jack Grealish he cannot guarantee him a place in his squad despite the midfielder's decision to represent England over the Republic of Ireland.
The Solihull-born 20-year-old turned out for Ireland's Under-17s as he qualified through his grandparents but his form for Aston Villa has seen him touted as a future England international.
Grealish turned down a senior call-up with Ireland for their friendly with England in June and pledged his allegiance to the Three Lions in a statement earlier this week.
He is unavailable for the European Championship qualification double-header against Estonia and Lithuania and, despite England having already qualified for next summer's tournament, boss Hodgson will not make special allowances to fast-track Grealish into his squad.
Meanwhile Jack Butland (Stoke City) and Jamie Vardy (Leicester City) have both made it into the England squad to face Estonia and Lithuania.
England squad to face Estonia and Lithuania:
Jack Butland, Joe Hart, Tom Heaton, Ryan Bertrand, Gary Cahill, Nathaniel Clyne, Kieran Gibbs, Phil Jagielka, Phil Jones, Chris Smalling, John Stones, Dele Alli, Ross Barkley, Michael Carrick, Adam Lallana, James Milner, Alex Oxlade-Chamberlain, Jonjo Shelvey, Raheem Sterling, Danny Ings, Harry Kane, Wayne Rooney, Jamie Vardy, Theo Walcott.