Nikki Sinclaire has conceded that she will not be re-elected as MEP for the West Midlands.
Ms Sinclaire won as a UKIP candidate in 2009, but left the party a year later, and set up the "We Demand A Referendum Now Party" in 2012.
Full results are yet to be announced.
Senior Liberal Democrat sources in both the West and East Midlands have told ITV News Central they fear a very bad night.
The party seems on course to lose both Phil Bennion in the West and Bill Newton-Dunn in the East, leaving them without a Midlands representative in Strasbourg.
Labour leader Ed Miliband says his party are ready for an Autumn by-election when candidates will compete for the Corby seat left by Conservative MP Louise Mensch.
The moment Labour returned to power in Derby. Their 4th gain of the night.
The 4th they needed to clinch victory.
For the Tories it was a grim night.
The former leader of Derby City Council admitted tax changes in the budget and issues such as Bombardier had hit them hard.