More than 50,000 spiders are being brought to the Ricoh Arena in Coventry by the British Tarantula Society later this month.
The event - which is said to be the 'Crufts' of the spider world, will take place on May 18.
Guests from across the world are expected to attend.
Spider-related items, such as tarantulas and equipment, will be on sale.
Ray Hale, vice chairman of the British Tarantula Society, said: “To be honest, it would be impossible to count how many spiders are on display but I think 50,000 is a conservative estimate."
“Visitors will be able buy a variety of arachnids, scorpions, stick insects, beetles and other insects throughout the day, meet other enthusiasts and seek advice from experts on the best way to look after their new pets.”
Eddie and Bernice Kozlowski from Nottinghamshire both developed swelling and sore itchy lumps after being bitten by a venomous spider - despite living for nearly 20 years in Australia, and escaping all the country's creepy-crawlies.
An elderly couple from Nottinghamshire are recovering at home after being bitten by what experts are calling ‘a cousin’ of Britain’s most venomous spider, the false widow.
Eddie and Bernice Kozlowski, who lived in Australia for 18 years, managed to catch the spider in their Gedling home on Sunday before it was sent to Nottingham Trent University to be analysed by experts today.
The couple have both suffered bites in the last three weeks, with Mr Kozlowski being bitten on his right arm. The two now have swelling and soreness and Mr Kozlowski says the pain after the bite was so severe he was unable to sleep.
The arachnid, believed to be a relative of the false widow (also known as steatoda bipunctata), is usually found in outbuildings but was captured by the pair down the side of their stairs.