A vintage Aston Martin DB5, previously owned by Paul McCartney, is expected to fetch up to £380,000 when it goes under the hammer.
The sports car, ordered by the Beatles star in 1964 on the eve of the band's world tour, will be auctioned in Battersea Park, London at 6.30pm today.
Peter Wallman, who will be co-running the bidding for RM Auctions, said the fame of both the car and the owner made it a particularly special item.
Mr Wallman said: "The Aston Martin DB5 is one of the most iconic cars in the world, driven by James Bond. And we're talking about one of the most iconic super groups of the 1960s.
"We've sold a couple of cars owned by Steve McQueen which have sold for eight, nine, 10 times what they would be worth if they were just regular cars.
"I'm not going to predict that happening here but if somebody collects Beatles memorabilia and just really wanted to have Paul McCartney's Aston Martin then it could go crazy."
The Aston Martin is one of 93 classic cars going under the hammer in the auction which is expected to bring in between £18m and £20m.
Sir Paul ordered the dark blue sports car in 1964 and had it fitted with a black leather interior, Motorola radio and its own record player.
He bought it weeks after The Beatles' appearance on The Ed Sullivan Show in America and the completion of filming A Hard Day's Night.
According to contemporary records, it then became a regular feature of his 1960's lifestyle.
Bandmate George Harrison, also owned a DB5 model, which sold for $464,000 in 2007.
Also on sale at the auction is a 1998 Ferrari F300 Formula One racing car used by Michael Schumacher and Eddie Irvine.
The most expensive car being sold is 1955 Mercedes-Benz 300SL Alloy Gullwing, one of only 29 ever produced, according to RM Auctions.
It is expected to fetch between £2.7m and £3m.