Bus lanes in Liverpool will be scrapped from today as part of a trial to reduce congestion.
Twenty-four lanes will be suspended for the next nine months.
Bus company Stagecoach believe the focus should be on creating more bus priority measures.
A Stagecoach spokesperson said: "Bus priority measures are vital in helping bus operators run more punctual and reliable services for our customers, and they are crucial to attracting more people out of their cars and on to bus travel."
"We believe the focus for Liverpool should be on more bus priority measures to help drive increased use of public transport - that is the most effective way to reduce pollution and congestion in the city."
Council bosses in Liverpool will begin a trial today to allow commuters to use the cities bus lanes.
All bus lane cameras will be immobilised and work will begin to removed signage.
Mayor of Liverpool, Joe Anderson, said:
"I have asked for this trial suspension so that we can explore what benefits, if any, bus lanes are bringing to our city. Keeping the city moving for our motorists, businesses, residents, commuters and visitors is absolutely vital, so it's important we take a proper look at this.
Some people have suggested to me that we shouldn't do this because the bus lanes generate income of £700,000-a-year for the council. But in my view it would be immoral to treat motorists as a cash cow.'"
The Green Party opposes the move on the basis is it discourages people to use public transport.