Isle of Man cyclist Mark Cavendish is out of the Tour de France, his team has confirmed.
It follows the shoulder injury he suffered on the first stage in Harrogate yesterday.
The pain of his injuries means he is no longer able to continue, a spokesman said.
It is the first time since 2008 - when he left prematurely to prepare for the Beijing Olympics - that Cavendish will not complete the Tour.
Isle of Man cyclist Mark Cavendish will learn today whether his Tour de France challenge will continue.
He crashed out of yesterday's opening stage and suffered an injury to his right shoulder which caused him "a lot of pain", his team Omega Pharma - Quick-Step Cycling said.
"I'm gutted about the crash," Cavendish said.
"It was my fault. I'll personally apologise to Simon Gerrans as soon as I get the chance.
"In reality, I tried to find a gap that wasn't really there.
"I wanted to win today, I felt really strong and in a great position to contest the sprint thanks to the unbelievable efforts of my team.
"Sorry to all the fans that came out to support - it was truly incredible."
Hospital tests showed Cavendish suffered ligament ruptures with an AC-joint dislocation, which causes him a lot of pain, his team said in a statement.
A final decision on his participation in today's stage will be taken this morning.
Thousands of spectators, including the Duke and Duchess of Cambridge, Prince Harry and Prime Minister David Cameron, were willing Cavendish on as the crash happened in Harrogate.
He came to grief in the town centre as his mother, Adele Towns, watched from the stands.
The VIPs looked as shocked as the masses of cycling fans crowded around the finish line when they heard the news over the speakers.
Cavendish was left on the ground clutching his shoulder after colliding with fellow competitor Simon Gerrans, injuring a joint between his right shoulder and collarbone.
The Isle of Man's Mark Cavendish's bid for the yellow jersey ended in a heap as he tumbled to the tarmac in a dramatic conclusion to the opening stage of the 101st Tour de France.
Cavendish was bidding for a 26th Tour stage win of his career in his mother's home town of Harrogate and a first yellow jersey, but collided with Australian Simon Gerrans in the final 500 metres and was seen clutching his shoulder and midrift as he rolled over the line.
Cavendish was later taken away from the Omega Pharma-Quick Step team area in an ambulance and transported to hospital.
Stars of Coronation Street and Emmerdale got into the Tour de France spirit by cycling 60 miles across the Pennines from the Rovers Return to the Woolpack.
The Tour de ITV celebrated the famous cycling event, which starts in Yorkshire on Saturday, and aims help two charities.
Sarah Rogers has this report:
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