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The colony of bees has now been safely moved across the road to Westminster Cathedral, where it will be looked after by beekeepers on top of the Catholic church's roof.
David Beamont, at the Victoria Business Improvement District said: "In Victoria there are over a dozen experienced beekeepers who have all been on an intense training course that focuses on the theory and practical training of urban beekeeping, successfully managing hives for nearly two years."
Ruth Duston, CEO of Victoria BID, said local businesses kept bees to boost the area's biodiversity, adding: "With a falling national bee population, the bees in Victoria play a key role in showing that London is a healthy, liveable city."
Tony Mann, a project manager at nearby John Lewis, who also happens to be a trained bee keeper, dashed over to the shop to help lead efforts to smoke the European honeybee colony out.
Tony Mann said: "We have either had a virgin Queen or an old Queen, she has left the nest and she has brought the warm and settled on the shop front. We are going to smoke them into a box and encourage them to stay there and we will remove them later today.
"They will go to whoever wants a hive. We need bees in London as much as we need them on the countryside. Bees are a keystone species. These are European honeybees."
A huge swarm of thousands of bees sent commuters running for cover near London's Victoria Station this morning. The insects covered part of Topshop's window as Londoners headed to work.
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The unusual nesting place was picked by the Queen bee, quickly followed by her devoted colony.