The Save Downhills campaign group said they were "disappointed but not surprised" their application was refused and were considering whether to appeal.
The group said in a statement: "Today's ruling shows that consultation during academy conversion is irrelevant.
"Parents' voices have been completely ignored. The law appears constructed to ensure that only one man, the Secretary of State, can make decisions about any school's future. Downhills is a happy, inclusive and improving school as the latest test results and Ofsted monitoring will show.
"We believe we are the victims of his wider political agenda. He publicly abused us as 'enemies of promise' and said he was 'marching towards the sound of gunfire'. We continue to believe Mr Gove has abused his power, denying parents and local communities their legitimate voice."
Here's how we reported on the Downhills academy row back in March.
The Department for Education has welcomed the High Court ruling over Downhills Academy.
Parents had argued that Downhills Primary was now thriving - and that turning it into an academy made no sense.
But, as he refused permission for a judicial review, Mr Justice Kenneth Parker said that it was a rational decision to turn it into an academy, in the light of the school's "egregious" past performance.
He said that it was clear that the Education Secretary "simply had no confidence" that the school would substantially improve if it remained within the control of Haringey Council.
On the other hand, he "believed firmly" that conversion to an academy would substantially improve performance.
Campaigners have lost a High Court battle to prevent a Tottenham primary school being turned into an academy.
Parents of students at Downhills primary accused Education Secretary Michael Gove of "riding roughshod" over their wishes, when he decided that the school must be removed from local authority control.
They had launched a bid for judicial review - but today that application was turned down.
Teachers, who have gone on strike in protest against plans to turn their school into an academy, were joined by parents as they demonstrated outside the school today.
Up to 40 parents attended the protest at Downhills Primary in Haringey.
Sarah Williams, 40, who has two sons at the school, said: