City of London police, who were supported by the Met, said 23 arrests were made during the "largely peaceful" eviction as bailiffs from City of London Corporation removed tents and equipment from outside St Paul's last night.
The action was taken five days after Occupy London was refused permission by the Court of Appeal to challenge orders evicting protesters.
Stuart Fraser, policy chairman of the City of London Corporation, said: "It is regrettable that it had to come to the need for removal but the High Court judgment speaks for itself. The site has now been cleared and the area is undergoing a deep clean."
"In the past few months, we have all been made to re-examine important issues about social and economic justice and the role the cathedral can play.
We regret the camp had to be removed by bailiffs but we are fully committed to continuing to promote these issues through our worship, teaching and Institute. The cathedral is open today and set aside for prayer and reflection. The cathedral is accessible to everyone.
The area currently cordoned off is for essential repairs to damaged paving.
Clergy are available throughout the day for pastoral care and support."