Farringdon nightclub Fabric reopens tonight.
The club had its licence revoked by Islington Council but reached an agreement with Scotland Yard and the council in November.
An inquest heard that a teenager who died after taking ecstasy on a night out at Fabric bought another pill inside the nightclub.
The nightclub has confirmed it will appeal Islington Council's decision to revoke its licence.
The DJ said the nightclub's closure signified the decline of London's diverse arts scene.
The £35,000-a-year role will be to "ensure London thrives as a 24-hour city" after the decision to shut the club.
Sadiq Khan said the club is an "essential part of London's nightlife" for many clubbers.