Police have issued CCTV images of a man and two younger women they are trying trace following the attacks.
In a statement, the Met Police said: "In the first incident, at approximately 0400hrs, the substance was sprayed at two men outside the Lightbox nightclub in South Lambeth Place, Vauxhall. This followed a verbal exchange between the two men and a group outside the venue.
"Following comments made during the exchange, this incident is being treated as a homophobic hate crime."A short time later, police were made aware of a third man who had also been sprayed in the face with a substance in the vicinity of the nightclub.
More top news
A bright and sunny day tomorrow, but feeling distinctly cool and chilly tonight.
An ITV News London investigation has discovered the inquest will now be held at Westminster Coroner's Court.
The family court finalised the adoption as the criminal proceedings were still under way. The couple have vowed to fight for their child.