In order to protect local badgers, the Hampshire and Isle of Wight Wildlife Trust will start to vaccinate the badgers across the region against bTB in July this year.
Badgers play a big part in the spread of Bovine tuberculosis (bTB) which can have a devastating effect on cattle and cattle farmers.
The Environment Secretary Owen Paterson announced earlier in the year that pilot badger culls will go ahead in June.
The Badger Vaccination Appeal has already raised over £39,000 towards initial costs of the programme but the appeal's target is £45,000.
Donations can be made via phone or cheque in the post to the Trust.