Mark Molesley netted a late winner as Conference side Aldershot knocked 2008 winners Portsmouth out of the FA Cup with a 1-0 victory at the Recreation Ground.
Sky Bet League Two outfit Pompey, who also reached the FA Cup final in 2010, were the victims of a giant-killing as the home side's battling performance was rewarded when Molesley scrambled the ball home after 81 minutes.
In a frantic opening to the first-round replay, the visitors could have taken the lead inside the first minute, but Chris Barker was on hand to clear Jack Whatmough's header off the line.
And the best chance of the opening 45 minutes fell to Portsmouth midway through the first half when Ricky Holmes did well on the left to dig out a cross before Jed Wallace's acrobatic volley was parried to safety by home goalkeeper Phil Smith.
Aldershot top scorer Brett Williams almost broke the deadlock after 71 minutes when his low strike was turned around the post by Paul Jones before Manny Oyeleke flashed a shot wide moments later.
Aldershot pressed forward and Jones had to be alert again to deny Dan Holman from 25 yards with 10 minutes remaining.
But the hosts got their reward from the resulting corner as Molesley bundled the ball over the line after an almighty scramble in the six-yard box to delight the home fans.
Aldershot manager Andy Scott said "I'm very proud of the team. We had a big crowd, on television, and the players took it in their stride.
"We play Rochdale at home next. It's an opportunity to get in the third-round hat and that's what we've been looking for."
Richie Barker has been sacked as manager of Portsmouth FC after only 20 games in charge.
The 38-year-old won only four of his games in charge of the League Two side which sits two points above the relegation zone.
Pompey take on Newport this Saturday. Academy boss Andy Awford is expected to be placed in temporary charge.
Pompey have appointed Richie Barker as their new manager, with Steve Coppell also arriving in a Director of Football role.
Barker, 38, arrives following an 18-month spell at Crawley. He guided them to a 10th-place finish in League One.
“This is a fantastic opportunity for me,” he said. “I’m very excited to be at this club with its great history. Everyone knows how passionate the people of Portsmouth are about their football club.”
Barker’s first managerial post was at Bury. He took over in April 2011 with eight games of the season remaining, winning six in succession to secure promotion from League Two.
Jed Wallace opened the scoring in the 20th minute, David Connolly made it two six minutes later and, after Andy Wright had been sent off, Patrick Agyemang wrapped up a 3-0 victory six minutes before the break.
Southend have made their best start to a season in 23 years and share top billing with Oxford.
The Shrimpers defeated Northampton 2-0 at Roots Hall today thanks to Anthony Straker (38) and Freddy Eastwood (62).
Oxford lead the table on goal difference after a 3-1 victory over Torquay.Striker James Constable was instrumental in Chris Wilder's side claiming their third league win in a row, scoring a superb 62nd-minute goal and laying on the third for fellow substitute Asa Hall (78).
Portsmouth suffered a sorry start to life in SkyBet League Two as they crashed to a 4-1 home defeat at the hands of Oxford. Pompey are considered one of the favourites for promotion and things looked promising for Guy Whittingham's relegated side.
They took a 25th-minute lead through Patrick Agyemang in front of a league-record 18,000 strong crowd at Fratton Park.
However, Oxford had not read the script and they turned things round to go into the break 2-1 up with goals from Deane Smalley and Alfie Potter.
Things got worse for Portsmouth just before the hour-mark when they lost Johnny Ertl to a red card and Oxford made the most of their numerical advantage to add salt to their opponents' wounds with further goals from Potter and Smalley.
For a full round-up of all today's action click here for in-depth match coverage from ITV Sport
Portsmouth Football Club claim they have sold more than 10,000 season tickets ahead of the new season in League 2. The tickets are double the usual attendance for a League 2 game. The club is the largest fan owned team in the UK.
Portsmouth mayor Frank Jonas will write a cheque for £1000 this afternoon to secure his community share in Portsmouth Football Club.
He will attend in his full formal attire and will be met by executive board members of the Pompey Supporters Trust and Portsmouth Community Football Club at 3:30pm.
Bournemouth, who were top at the start of the day, could only draw 0-0 at Tranmere but were promoted to the Championship as runners-up.
At the other end, Scunthorpe had their relegation to the fourth tier confirmed after Colchester defeated Carlisle, rendering the Iron's 3-1 win over Swindon futile.
It was a frantic finish for Scunthorpe with all four goals scored in the last seven minutes, including two in stoppage time, but it proved not to be enough.
Swindon took the lead in the 83rd minute through James Collins but Scunthorpe levelled four minutes later through Michael Collins.
Portsmouth, meanwhile, finished their season with a 3-2 defeat by Shrewsbury.
Connor Goldson, Joe Jacobson and Akwasi Asante gave Shrewsbury a 3-0 lead before Ashley Harris and Patrick Agyemang scored for Pompey, but it was not enough to finish their disappointing campaign with a final victory.
A late goal at Elland Road saw 10-man Brighton beat nine-man Leeds to secure a play-off place for Gus Poyet's side in an ill-tempered match.
Will Buckley opened the scoring for Brighton in the 10th minute, with Leeds midfielder Rodolph Austin shown red shortly after.
Brighton's Inigo Calderon was sent off midway through the second half for a challenge on El-Hadji Diouf, who scored the resulting penalty before being sent off himself after appearing to abuse the away fans following the goal.
Jose Ulloa had the final word as he headed a late winner for Brighton.
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Relegated: Telford, Ebbsfleet, Barrow, Stockport.