Campaigners fighting the new high speed rail line from London to Birmingham, Leeds and Manchester have launched an emergency plea for £100,000 to pay for a High Court appeal.
They lost all but one Judicial Review cases two weeks ago claiming consultation on the £33bn scheme was not carried out correctly by the Government.
The case they won was in connection with consultation over property consultation which the court rules was "unlawful" and will now be held again.
Campaigners will now challenge eight other rulings they lost but need to raise a substation sum of money to help pay for the appeal.
The line runs through parts of Oxfordshire and Buckinghamshire and campaigners say it has no business case and will ruin the countryside.
HS2 say it will create 100,000 jobs, benefit the economy and they are taking into account the environment.
They say the action will not delay the project.