Two men were injured and three horses died after a trailer carrying 10 of the animals overturned on the A303 eastbound carriageway, after colliding with a lorry.
Seven were sedated and removed by vets and expert animal rescuers but one was killed in the crash and two had to be put down.
Hampshire Fire and Rescue Service said the drivers of both vehicles were not seriously hurt.
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Samantha Jefferies and husband Clive had been undergoing fertility treatment when he died suddenly, aged 51, of a brain haemorrhage.
Wednesday afternoon's weather for the west of the region
Wednesday afternoon's weather for the east of the region