Analysis of division lists showed that no Conservative MPs backed the Labour motion to postpone the 3p fuel duty rise in January.
The Government has defeated a Labour motion to postpone the 3p fuel duty rise in January by 234 votes to 282 - a majority of 48.
But it means Labour can now go on the airwaves saying the Tories and Lib Dems voted against freezing petrol prices in 2013 (until the mini-budget on December 5)
82% of people surveyed for the ITV News Index by ComRes say they oppose a rise in fuel duty.
73% of those asked said they would be angry if a fuel duty rise goes ahead.
Four in five of those surveyed think a fuel duty rise will make the Government look out of touch and two-thirds believe a rise will make a material difference to their household’s income.
Despite being a staunch opponent of a rise in fuel duty costs, Conservative MP Robert Halfon will vote later against a Labour motion calling for January's 3p per litre rise to be delayed.
He told ITV News his Harlow constituents would understand his course of action is still geared towards keeping the cost down.
Fair Fuel UK campaigner and motorist presenter Quentin Willson has told ITV News 35,000 jobs will be saved if the Chancellor chooses, as expected, to not pursue the 3p fuel duty rise.
The Chancellor last announced a fuel duty freeze back in August, a move that Treasury figures suggest will have cost the Exchequer approximately £550 million.
But officials said that loss would be recovered by underspending in Government departments.
Figures in the Autumn Statement are expected to show how the fuel duty freeze has been paid for.
There's nothing official yet, but it's "looking" like the Chancellor will freeze fuel duty.
Ahead of tonight's vote, Tory rebels believe George Osborne has been listening to their concerns and will now freeze the duty for another three to six months.
Prime Minister David Cameron's official spokesman earlier declined to comment on whether the Government might back down on the 3p fuel duty rise.
He said the controversial rise was a matter for the Chancellor.
– PM's spokesman
There are a series of planned rises in fuel taxes programmed in and those will generate revenue which will help bring the deficit down. But what the Government has sought to do thus far is listen to the concerns of motorists and, where it can, delay or cancel those planned rises.
Decisions on tax and duties are a matter for the Chancellor. The Government obviously recognises that the cost of petrol is a significant part of day to day spending and that is why (it) has listened to the views of consumers and motorists and acted over the past two years by delaying or cancelling planned rises.
Shadow chancellor Ed Balls said George Osborne had been making "nods and winks" on fuel duty over the past 24 hours to dissuade Tory MPs from joining Labour in voting against the 3p rise today.
– Ed Balls, speaking to BBC Radio 4's World At One
I would say to Conservative MPs, I wouldn't take a nod or a wink from this Chancellor as sufficient. I would want to make a clear statement to my constituents - this is the wrong thing to do and I am going to vote with Labour.
Cancelling the Fuel Duty3prise-this is the time for determined, patient lobbying of Chancellor, not parliamentary theatre backing Ed Balls