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Labour leader Ed Miliband says investors would think twice about whether or not to put their money into Britain if the country thinks about leaving the EU.
Speaking this morning during his radio phone-in show on LBC, the Deputy Prime Minister Nick Clegg said years of guesswork and uncertainty over Europe needed to come to an end.
Vince Cable will urge David Cameron not to take a "dangerous gamble" with the national interest today as the Liberal Democrats mount pressure over Europe.
On the eve of the Prime Minister's expected announcement of a future referendum on UK membership of the EU, he will reinforce warnings the strategy risks damaging economic recovery.
Any effort to negotiate large-scale exits from existing EU powers would make it "next to impossible" to safeguard Britain's best interests in the single market, the Business Secretary will suggest.
Labour is expected to accuse the Prime Minister of "sleepwalking" towards British exit from the European Union later today.
"The real tragedy of tomorrow's speech is that David Cameron's party won't let him address the undoubted need for change in the EU in a sensible way," Shadow Foreign Secretary Douglas Alexander will say.
"We have a Prime Minister who simply cannot reconcile the demands of his party with the needs of the country.
"The gap between the minimum the Tories will demand and the maximum our European partners can accept remains unbridgeable.
"For many in his party, getting David Cameron to commit now to an in/out referendum is not about securing consent. It is about securing exit.
"And we will have a British Prime Minister sleepwalking towards exit, knowing he is letting down the national interest, but too weak to do anything about it."
More than 25 Conservative MP’s have submitted a letter to David Cameron prior to his Europe speech on Friday urging him to offer a "positive vision" of British leadership in Europe.
The group wants Mr Cameron to say that rather than leaving the EU, it is in Britain’s national interest to lead it and that official Conservative pro-European policy still stands.
The UK Independence Party is enjoying its "highest level of support" among voters, according to the latest survey, as the Prime Minister prepares to make a keynote speech on Britain's role in Europe.
Polling company Ipsos Mori's January Political Monitor found 9% of those certain to vote in the General Election said they would put a cross next to Ukip, a two point rise since last month.
It placed Nigel Farage's party ahead of the Liberal Democrats, who fell a point to 8%.
Mr Farage today said David Cameron, who will speak in the Netherlands on Friday, was trying to "deflect" the issue of Europe for five years but predicted that the debate would be decisive in securing UK's exit.
The eurosceptic MEP told the European Parliament: "The longer this debate goes on, the more likely the UK is to leave the European Union, and this debate will not stop."
Latest ITV News reports
Centrica's boss warns an EU referendum risks "total destabilisation" for businesses; the Next chief suggests that view is "scaremongering."
There will be sympathy, yes. But will they agree with him? No.