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The driver, a 42-year-old female ambulance technician, and the patient, an 88-year-old man, were pronounced dead at the scene.
A male crew member and a male relative of the patient remain in hospital both in a serious condition.
Police are continuing to appeal for witnesses following a collision involving an ambulance and a car in the New Forest.
A driver arrested in connection with the incident on the A337 yesterday morning has been bailed until July 29.
The 25-year-old, who was driving a blue Seat Alhambra, was arrested on suspicion of causing death by driving without due care and attention.
Both the car and the ambulance were heading north on Lyndhurst Road when, for reasons yet to be established, the ambulance came off the road and hit a tree.
A motorist remains in police custody after an ambulance driver and an elderly patient were killed when an ambulance crashed into a tree in Hampshire.
The car involved in the incident, a blue Seat Alhambra, was also travelling north, according to police. The 25-year-old male driver, from Brockenhurst, was uninjured.
Inspector Kirsty Shannon, of Hampshire Police, said:
Hampshire Police have asked for witnesses to come forward after an ambulance driver and an elderly patient were killed when the ambulance hit a tree in Hampshire.
Paul Jefferies, area Manager at the South Central Ambulance Service has said it is a "sad day", after a paramedic and an elderly patient were killed when an ambulance hit a tree in Hampshire.
Mr Jefferies added that the service would continue to work with Hampshire Police to help their investigation:
A motorist has been arrested after a paramedic and an elderly patient were killed when an ambulance crashed into a tree. In a statement South Central Ambulance Service said:
Two other cars were involved in the incident apart from ambulance.
Police will not give further details at this stage and will not identify the people involved for at least 24 hours.
Gwyn Williams was over taken by the ambulance shortly before the crash and told ITV news what he saw:
He had been driving along the A336 between Brockenhurst and Lyndhurst in the New Forrest.
Latest ITV News reports
A motorist has been arrested after a paramedic and an elderly patient were killed when an ambulance crashed into a tree in Hampshire.