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Prince Harry shared a tent with Captain Ibrar Ali, 36, who lost his right arm in a roadside bomb blast in 2007, and Major Kate Philp, who chose to have her left leg amputated after her Warrior armoured vehicle struck an IED (improvised explosive device) in Musa Qala in Helmand Province in 2008.
Maj Philp, from Worcestershire, said Harry was a "good extra pair of hands" during the training exercise.The 35-year-old Royal Artillery officer said having the prince with the team was "great".
The Walking with the Wounded team practised using cross trainers to simulate skiing for two hours at a time in the cold chamber.
In the cold chamber, the team will practice their routine for the South Pole using cross trainers to simulate skiing for two hours at a time before breaking for around 10 minutes and repeating this activity for 12 hours when they will set camp for the night.
In the South Pole the team will spend around four hours a day making and breaking camp so it is important that it is rehearsed to save time during the trek.
After coming out of his first visit to the cold chamber Prince Harry was asked how he had found the conditions, "cold" he said. Joking with the waiting press pack, he added: "it's your turn next."
Over a 20-hour stay the prince joined the Walking With The Wounded team as they prepare for their South Pole trek.
Prince Harry has been practising how to build and dismantle the tents he will use during his trip to the South Pole with Walking With The Wounded.
The team will race 335km to the South Pole in November.
Prince Harry, Patron of Walking With The Wounded South Pole Allied Challenge, is taking part in a 24 hour cold chamber training Exercise with the British team.
He has joined the team inside a special chamber which will simulate the extreme conditions they will face in the South Pole.
The Prince will test equipment and practice the routine tasks such as setting up the camp and skiing.
The chamber has a variable temperature and wind controls replicating the -60 degrees the Prince would face in the South Pole.
Prince Harry is to take part in a Cold Chamber training exercise with the British Walking With The Wounded South Pole Allied Challenge team today.
The operation aims to simulate the extreme conditions Harry and the team will face when the trek to the South Pole in November 2013.
In the specially-designed chamber, participants will use cross-trainers to ape the cross-country skiing necessary to reach the team's goal later this year.
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Prince Harry took part in 24-hour cold chamber training with the British Walking With The Wounded South Pole Allied Challenge team.