Alex Oxlade-Chamberlain is to undergo a scan to determine the severity of the injury he suffered against Barcelona on Tuesday night.
The 22-year-old Arsenal midfielder suffered suspected knee and ankle damage in the 2-0 Champions League defeat by the Spanish giants.
There are fears that the England international faces a lengthy spell on the sidelines, meaning he would miss the friendly matches against Germany and Holland next month.
But Gunners manager Arsene Wenger has so far only ruled him out of Sunday's trip to Manchester United.
Alex Oxlade-Chamberlain has suffered a setback in his recovery from a hamstring injury, ruling him out of Arsenal's Barclays Premier League clash at West Brom.
The England forward, who suffered the injury in the Capital One Cup loss to Sheffield Wednesday, is also expected to miss the key Champions League clash with Dinamo Zagreb next Tuesday.
Arsenal boss Arsene Wenger said: "He had a slight little setback on Monday but we checked by scan and there is nothing wrong but he is still a fraction short for Saturday."
England manager Roy Hodgson said Alex Oxlade-Chamberlain will "without a shadow of a doubt" make the World Cup squad after picking up an injury in the draw against Ecuador.
It looked as though the Arsenal midfielder's World Cup dreams were over after he picked up a knee injury on Wednesday.
But the England manager, who was speaking after the 0-0 draw with Honduras tonight, said: "It's time for the real thing now.
"Everyone is fit. Oxlade-Chamberlain is making good progress. I think he'll make the tournament without a shadow of a doubt."
Alex Oxlade-Chamberlain's World Cup dreams are in serious doubt after he suffered a knee injury in England's ill-tempered 2-2 draw against Ecuador in Miami.
Oxlade-Chamberlain had to come off in the 63rd minute of England's stalemate after being injured in a tangle with Ecuador's Carlos Gruezo.
England manager Roy Hodgson has said that the player will have a scan on the injured knee in the morning.
Roy Hodgson is set to give Andy Carroll his Euro 2012 chance against Sweden tonight, according to the Daily Mail.
Carroll will partner Danny Welbeck upfront as Alex Oxlade-Chamberlain drops to the bench.