Boris Johnson vowed to "eat the 'Kippers for breakfast" as he sought to unite Tories and lambasted those who had defected to Ukip.
Mr Johnson hit back at "quitters and splitters" and told activists that victory in next year's general election was "within our grasp" as long as the party remained united.
To loud cheers the Mayor of London said:
Just before I begin can I check that we are all proud Conservatives? Proud of the oldest and most successful party in all the Western democracies? Do we intend to fight the next election under the Conservative banner and no others? Are there any quitters or splitters? Anyone feeling a bit yellow around the edges - like a kipper?
Only the Conservative could deliver reform to the UK's relationship with the EU, he said. Comparing the successful fight against Scottish independence with the imminent by-election battles to retain the seats given up by defecting MPs Douglas Carswell and Mark Reckless, he said:
That's our new fisheries policy, folks. First chuck Salmond overboard and then eat the 'Kippers for breakfast. We will fight them on the beaches of Clacton and of Rochester and Strood as well.
London Mayor Boris Johnson has defended the Chancellor's reforms to welfare.
Speaking during a visit to a factory in Newcastle-under-Lyme, he said:
If you want to have the cash to pay for the whole of society, then you need the economy to be going gang-busters.
The most important thing for us in the UK economy is you've got to reform welfare and it's quite right that you should be looking at ways of improving how it's spent, but you've got to get the economy moving because that's the way to pay for the whole she-bang.
David Cameron has said he would like Boris Johnson in his cabinet after the next General Election, calling him "a star".
He told The Andrew Marr Show, "He's got to finish his term as Mayor of London, I know that's what he wants to do.
"But once he's finished that then, you know, he's a star and as I've always said, I want to have the stars in the team."
Boris Johnson has made an impassioned plea to keep the UK together, warning Scottish independence would be "an utter catastrophe for this country".
"We will all have lost a way of thinking about ourselves, a way of explaining ourselves to the world," the Mayor of London wrote in the Daily Telegraph.
"We are on the verge of trashing our global name and brand in an act of self-mutilation that will leave our international rivals stunned, gleeful and discreetly scornful."
A co-ordinator for the Back Heathrow campaign, which is calling for expansion of the airport, has called Boris Johnson's candidacy a "slap in the face".
Boris Johnson's selfish plan to stand in the same borough as Heathrow is a slap in the face to tens of thousands of people in west London.
These people rely on Britain's hub airport and the Mayor wants to close it which would result in more than 100,000 people losing their jobs including many in Uxbridge and South Ruislip.
Heathrow's bogeyman may win the selection battle but he won't get his way.
Labour has said that Boris Johnson's bid to become a Tory MP is "confirmation of how weak David Cameron is and how out of touch the Tories are".
The party's London spokesman Sadiq Khan accused Conservative politicians of "jockeying for position in a future leadership contest" instead of tackling the "cost of living crisis".
Boris Johnson is seeking to become Conservative candidate for Uxbridge and South Ruislip at next-year's general election, his spokesman has confirmed.
The Mayor of London confirmed he wanted to stand for Parliament earlier this month, with David Cameron saying it was "great news" that one of his "star players" was returning to the Commons.
Mr Johnson said he hoped to "make his case" that he was the best person to represent the constituency, held by Sir John Randall with a majority of 11,000 at the last general election.
"I'm sure there will be plenty of excellent candidates and I hope very much to make my case to the association," he told the Evening Standard.
Boris Johnson has said tougher laws are needed to combat the threat of British extremists who leave to fight for the Islamic State.
Writing in the Daily Telegraph, Mr Johnson said control orders should be brought back immediately for the most serious cases.
The Mayor of London said those who travel to fight for the terrorist group should be stripped of their citizenship - echoing calls from senior Tory MP David Davis.
Johnson said there should be a "swift and minor change to the law" so that there is a "rebuttable presumption" that those who have travelled to Iraq and Syria, without notifying the authorities, have done so for a "terrorist purpose".
Johnson warned that unless Britain acts to "close down" the Islamic State, the "tide of terror will eventually lap at our own front door."
More than half of voters in a London constituency which is seen as a target for Boris Johnson would vote for him if he chose to stand there in 2015, according to a poll.
A survey conducted in Uxbridge and South Ruislip after the London Mayor finally confirmed his desire to return to the Commons showed he could significantly boost Tory support.
But a third of those who backed him also said he should quit City Hall if elected - despite Mr Johnson insisting he could see out the final year of his term.
The poll of 1,000 people in the seat being vacated by former deputy chief whip John Randall was carried out for Tory donor and former party vice chairman Lord Ashcroft.