Gareth Bale is likely to be out for a month after the Real Madrid forward sustained a calf injury in Sunday's El Clasico loss to Barcelona.
Real confirmed that Bale injured his left calf - diagnosed as a "grade two injury" - with reports suggesting the Welshman will miss around four weeks of action.
The injury means Bale is set to miss both of Real's Champions League semi-final legs against Atletico, on May 2 and 10 respectively.
Following the tests carried out on Gareth Bale by the Sanitas Medical Services, the player has been diagnosed with a grade 2 injury in his left calf. His recovery will continue to be assessed.
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Barcelona coach Luis Enrique is optimistic his side can pull off another stunning comeback to advance in the Champions League.
The Catalan side looked down and out after losing the first leg of their last-16 tie 4-0 against Paris St Germain in February, but produced a remarkable 6-1 second-leg triumph last month to reach the quarter-finals of Europe's premier club competition.
They gave themselves another mountain to climb as they suffered a crushing 3-0 loss to Juventus in Turin last Tuesday in the first leg of the last-eight meeting, but Enrique has warned Barca should not be written off just yet.
We will try right until the end. Those who experienced the night against PSG at Camp Nou will have another chance to enjoy a historic evening. Right until the 95th minute, we have to be united.
We have to get the fans involved in our game and our intensity. We need Camp Nou to bounce to experience another special evening.
Many results at home and the recent comeback against PSG are statistics that invite optimism. We have to create a collective crazy atmosphere so our players can perform at their best.
We need to intimidate a team like Juventus to the maximum. If we score the first goal, Camp Nou will get the second and the third will come by itself.
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Manuel Neuer suffered a broken foot in Bayern Munich's Champions League defeat at Real Madrid and looks set to miss the rest of the season.
The goalkeeper received a fracture to his left foot in extra-time of the 4-2 loss at the Bernabeu, which condemned the visitors to a 6-3 aggregate defeat in the quarter-final.
Neuer, who last month underwent what was described by the club as a minor operation on his left foot, picked up this latest injury during the build-up to Cristiano Ronaldo's third goal, which put Madrid 3-2 up on the night.
Initial tests have revealed the damage, with Bayern saying in a statement he would "undergo a thorough examination on arrival in Munich prior to a decision on the appropriate treatment".
Chief executive Karl-Heinz Rummenigge said at the team's post-match banquet in Madrid: "Manuel Neuer has injured himself badly, he will be out for eight weeks."
That diagnosis would rule him out of the remaining five matches of the Bundesliga season - not a huge blow given his club boast an eight-point lead at the top of the standings.
More significantly, he could miss the DFB-Pokal semi-final against Borussia Dortmund later this month and a possible final against either Borussia Monchengladbach or Eintracht Frankfurt on May 27.