Juventus are 3-0 up from the first leg and Barcelona will have to become the first ever team to overturn that deficit to reach the Champions League semi-finals.
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Barcelona coach Luis Enrique is optimistic his side can pull off another stunning comeback to advance in the Champions League.
The Catalan side looked down and out after losing the first leg of their last-16 tie 4-0 against Paris St Germain in February, but produced a remarkable 6-1 second-leg triumph last month to reach the quarter-finals of Europe's premier club competition.
They gave themselves another mountain to climb as they suffered a crushing 3-0 loss to Juventus in Turin last Tuesday in the first leg of the last-eight meeting, but Enrique has warned Barca should not be written off just yet.
We will try right until the end. Those who experienced the night against PSG at Camp Nou will have another chance to enjoy a historic evening. Right until the 95th minute, we have to be united.
We have to get the fans involved in our game and our intensity. We need Camp Nou to bounce to experience another special evening.
Many results at home and the recent comeback against PSG are statistics that invite optimism. We have to create a collective crazy atmosphere so our players can perform at their best.
We need to intimidate a team like Juventus to the maximum. If we score the first goal, Camp Nou will get the second and the third will come by itself.
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Manuel Neuer suffered a broken foot in Bayern Munich's Champions League defeat at Real Madrid and looks set to miss the rest of the season.
The goalkeeper received a fracture to his left foot in extra-time of the 4-2 loss at the Bernabeu, which condemned the visitors to a 6-3 aggregate defeat in the quarter-final.
Neuer, who last month underwent what was described by the club as a minor operation on his left foot, picked up this latest injury during the build-up to Cristiano Ronaldo's third goal, which put Madrid 3-2 up on the night.
Initial tests have revealed the damage, with Bayern saying in a statement he would "undergo a thorough examination on arrival in Munich prior to a decision on the appropriate treatment".
Chief executive Karl-Heinz Rummenigge said at the team's post-match banquet in Madrid: "Manuel Neuer has injured himself badly, he will be out for eight weeks."
That diagnosis would rule him out of the remaining five matches of the Bundesliga season - not a huge blow given his club boast an eight-point lead at the top of the standings.
More significantly, he could miss the DFB-Pokal semi-final against Borussia Dortmund later this month and a possible final against either Borussia Monchengladbach or Eintracht Frankfurt on May 27.
Cristiano Ronaldo praised the winning mentality of Real Madrid after his hat-trick helped defeat Bayern Munich 4-2 following extra time at the Bernabeu.
A Robert Lewandowski penalty put Bayern ahead on the night but a diving header from Ronaldo with 14 minutes left looked to have been enough to send the Spanish side through, only for a bizarre own goal from Real captain Sergio Ramos to level things up at 3-3 overall.
Bayern saw midfielder Arturo Vidal sent off for what looked a harsh second caution to go into extra time with just 10 men.
Ronaldo's second goal in the 105th minute saw the forward become the first player to score 100 Champions League goals, while he completed his hat-trick four minutes later - Marco Asensio added the icing on the cake to make it 6-3 on aggregate.
We are just winners. Making six goals against a team like Bayern is not easy and we deserve to move through to the semi-finals.
We played very well, obviously I am very happy to score all three goals, but the team has been great.
We knew we had to play very well because Bayern can score goals from anywhere on the pitch and proved to be an excellent team, but Real Madrid is Real Madrid.
In the first half we had many opportunities and we could score a goal or two, also in the second half too.
Yes, we conceded two goals, but Real Madrid is used to this setback, we won and we are all very happy.
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