Chris Smalling has been forced out of the England squad through injury, leading Middlesbrough defender Ben Gibson to receive his first call-up.
Gareth Southgate was already facing a defensive headache for Sunday's World Cup qualifier against Lithuania as Gary Cahill, the skipper in Wednesday's 1-0 defeat in Germany, was suspended and Phil Jones withdrew through injury on the eve of the friendly.
The Manchester United defender sustained a toe injury in what appeared to be a training ground duel with team-mate Smalling, who on Friday left the squad with fitness problems of his own.
The centre-back has returned to Manchester for treatment on the unspecified issue, with Southgate turning to uncapped Middlesbrough defender Gibson.
The 24-year-old was a regular for the England Under-21s during Southgate's reign and has been linked with a variety of clubs having impressed during Boro's Premier League campaign.
Southgate had not been planning to call up any reinforcements for Sunday despite the absence of Cahill and Jones, but Smalling's setback has forced his hand.
"We'll just assess over the next 24 hours if we have picked up any other knocks," the England boss said in Dortmund.
"I am not aware of anything significant at the moment so if that is the case we wouldn't."
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Adam Lallana feels Dele Alli can be a "special" player for England, provided he is given time to fulfil his promise.
Lallana and Alli were pushed up as supporting attackers in Wednesday's 1-0 friendly loss in Germany, turning in eye-catching performances in Gareth Southgate's re-jigged 3-4-2-1 system.
Both players had wonderful chances to put the visitors ahead before half-time in Dortmund, Lallana hitting the post after a fine solo run and the Tottenham man denied by Marc-Andre ter Stegen after going clear in the box.
Although he was frustrated not to do better on that occasion, Alli's all-round performance, full of neat touches and tricks, was hugely promising for a player still three weeks shy of his 21st birthday.
And Lallana feels it was a sign of things to come if Alli is allowed to develop.
Dele is special. I love the way he goes about his business: no fear, he's brave.
That's what makes him 'him'. All players have different attitudes that make them what they are and that's why he is unique and a special talent.
But people still need to be patient with him. He is still a young boy and performing how he does is way above his years. He is not even 21 yet.
It is important that we don't get carried away.
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Burnley defender Michael Keane says playing for England was always his dream - even when he was turning out for the Republic of Ireland at youth level.
Keane has been a regular around the Three Lions squad since Gareth Southgate took over as manager and earned his first cap in Wednesday's 1-0 defeat to Germany in Dortmund.
As well as ending the Clarets' 43-year wait to see an outfield player capped, it was the first step to a successful international career for the highly-rated Manchester United graduate.
Although born in Stockport, Keane was eligible for the Irish side through his father and wore green at Under-17 and Under-19 level, only to pledge for England in 2012.
And Keane has admitted he had something to prove after leaving Old Trafford.
I always wanted to play for England but when I was younger I was not at the level required so I thought it best to go and get some experience at international level. That is what I did.
When I got a call saying they wanted me I did not think twice. I was thankful to Ireland to play but England was my country. There was never any doubt.
My dad was born in Ireland but moved to England when he was nine, he's over the moon.
I have been striving to get here for the last year or two having come through the Under-21s so to finally do it, on a stage like this, against the world champions is a dream come true.
I felt comfortable. There were a few nerves and butterflies as you would expect but it helped me playing alongside experienced players. They were talking through me the game the whole time.
I always thought if I am playing well in the Premier League I would have a chance. I was always confident in my ability... I always thought I would make it at United.
A few changes in manager did not help and when I left I felt I had a point to prove and it made me work that bit harder. I feel maybe I have proved it.
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Gareth Southgate says he was pleased with how his players performed following England's 1-0 defeat to Germany last night.
It was Southgate's first match as permanent boss of the Three Lions and his side showed pride, promise and potential.
"I am not somebody that is over-positive if you have been defeated, but I have to be really pleased with the way the players have played individually and in terms of the tactical system and the way that worked," Southgate said.
"Their ability to adapt quickly to that I thought was excellent.
"Up until the goal I thought we were really the better side. Germany then had a little spell when they controlled it and obviously we put some of our younger players on.
"That was a good learning experience for them, but I am very pleased with what we learned, the manner of the performance.
"And the one bit we missed was the finish. We should have had the game won, really, but I am very pleased with what we did."
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Gary Cahill says he has confidence in his England team mates ahead of their clash with Germany tonight.
The Chelsea defender, who has made 38 appearances in all competitions this season, will wear the captain's armband in Dortmund tonight.
When asked by the media if he was intimidated by rivals Germany, he said: "Me, personally no.
"Certainly respectful of the history of what they've done but man for man I'm confident in the squad we've got and players we have when I'm going out, like against Spain.
"I'm respectful of what they've done and achieved as players.
"I'm respectful that they're top Champions League players and win trophies but I'm confident in my own team mates. So no. not really."
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