US Open champion Dustin Johnson has become the latest golfer to withdraw from the Olympics over fears of the Zika virus.
Several players including world number one Jason Day and four-time Major winner Rory McIlroy have already pulled out of Rio 2016 citing worries over the virus and Johnson has become the first American to do so.
Zika is a mosquito-borne virus which has been linked to defects in newborn babies.
"As an athlete, I can think of no greater honor than representing the United States in the Olympic Games," Johnson said in a statement released by his agent David Winkle.
"However, after much careful consideration and discussion with both my family and my team, I have made the decision to withdraw from the 2016 Olympic Games."
Jason Day has pulled out of the Rio Olympics because of concerns over the zika virus.
The Australian's decision to pull out of the Games follows that of fellow golfer Rory McIlroy.
In a statement released on twitter, he said: "The reason for my decision is my concerns about the possible transmission of the zika virus and the potential risks that it may present to my wife's future pregnancies and to future members of our family.
"Medical experts have confirmed that while perhaps slight, a decision to compete in Rio absolutely comes with health risks to me and to my family.
"While it has always been a major goal to compete in the Olympics on behalf of my country, playing golf cannot take precedent over the safety of our family."
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Danny Willett has told ITV News he is looking forward to spending time with his wife and new baby boy, after touching down at Manchester airport following his dramatic US Masters triumph.
Warning: this report contains flashing images
Video report by ITV News' correspondent Damon Green
The Sheffield-born golfer is the first Englishman to win the coveted green jacket for 20 years - and only the second to do so.
Danny Willett said his success may change things "a little bit" in normal walks of life.
US Masters champion Danny Willett has said he is looking forward to spending time with his family - including changing nappies - after returning to the UK.
Willett - the first Englishman to claim the green jacket since Nick Faldo in 1996 - returned home on Tuesday and said he hopes he will be able to keep some normality in his life despite his new-found fame.
The 28-year-old from Sheffield said it had been a "tough week" being away from his wife and newborn son and he was "really looking forward to getting back".