Former England footballer Michael Owen's prize horse is to miss Royal Ascot's blue-ribbon race after picking up an injury.
Brown Panther was third-favourite for the Gold Cup, but Owen announced on Twitter that he was out of the running.
Absolutely gutted to announce that Brown Panther injured his near fore leg this morning and is out of The Ascot Gold Cup on Thursday.
Many Clouds has won the Grand National at Aintree for trainer Oliver Sherwood and last year's winning jockey Leighton Aspell.
Successful aboard Pineau De Re 12 months ago, Aspell was never too far off the pace and Many Clouds, winner of the Hennessy Gold Cup at Newbury earlier in the season and sixth in the Cheltenham Gold Cup, jumped with aplomb throughout.
For a long way it appeared Shutthefrontdoor could provide Tony McCoy with a fairytale second National triumph before his retirement, but he eventually weakened into fifth place.
Many Clouds tired from the elbow, but had enough in the tank to hold off Saint Are, with Monbeg Dude third and Alvarado fourth.
Coneygree has won the Cheltenham Gold Cup, becoming the first novice to win the race since 1974.
Rugby Walsh sealed his third Cheltenham victory on the opening day of the festival after winning the Champion Hurdle with Faugheen.
Douvan gave Willie Mullins and Ruby Walsh the perfect start to the four-day Cheltenham Festival when running out a smooth winner of the Sky Bet Supreme Novices' Hurdle.
Walsh brought the 2-1 favourite to lead before the final flight and Douvan bounded up the hill to score by four and a half lengths.
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Jockey Tony McCoy today announced he is to retire from racing at the end of the season.
Just minutes after Mr Mole gave McCoy his 200th winner of the season as Sire De Grugy unseated three out in an incident-packed Betfair Price Rush Chase at Newbury, the champion jockey told Channel 4 Racing of his intention to stop riding.
Not seen since following up his Champion Chase success at Sandown last April, the Gary Moore-trained Sire De Grugy was sent off at odds-on but made a bad mistake four from home before parting company with Jamie Moore at the next.
That left a simple task for Mr Mole, who will test his credentials at the top level in the two-mile championship next month.
All that was overshadowed, however, when McCoy revealed his retirement plan.
"It's going to be the last time I ride 200 winners," he said.
"Having spoken to Dave (Roberts, agent) and JP McManus, I am going to be retiring at the end of the season."
McCoy said: "I want to go out at the top, I want to go out as champion jockey and it will be my 20th year if I can win the jockeys' championship."
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Irish Flat jockey Danny Grant has been suspended for six months after he failed a drugs test at the Curragh on March 23.
Grant was officially handed a two-year riding ban by the Irish Turf Club, but the last 18 months of the disqualification period was suspended under the assumption he does not fail another test.
He must also remain available for further drugs testing during the entirety of the suspension.
Grant tested positive for metabolites of cocaine at the Curragh on the first day of the Flat turf season in Ireland.