Jose Manuel Barroso has said that the £1.7 bn surcharge levied against the UK, "should not come as a surprise," as it was calculated from figures provided by the UK.
The European Commission President was answering a question from ITV News Europe Editor James Mates, on David Cameron's claims the payment was "not acceptable".
When pressed on what would happen if the UK does not make the payment on 1st December, when it is due, Mr Barroso said: "I cannot now speculate on non-payment."
EU Commission president Jose Manuel Barroso wrote of Yulia Tymoshenko's release from prison in Ukraine:
The president of the European Commission has flatly rejected any prospect of member states capping migration from fellow EU-member countries.
Jose Manuel Barroso said freedom of movement for labour was a "fundamental principle" of the EU single market, with a cap on immigration being a "complete contradiction" to that principle.
European Commission President Jose Manuel Barroso has accused those seeking new limits to migration between EU member states of "chauvinism" and "scaremongering".
Mr Barroso did not mention any countries by name, but his comments are likely to be seen as a rebuke to the UK after Prime Minister David Cameron's repeated warnings about migrants from eastern Europe coming to Britain to claim welfare.
Speaking to the European Parliament in Strasbourg, Mr Barroso issued a strong defence of free movement for EU workers, and warned the issue was "in danger of being subject to all kinds of populist rhetoric rather than objective assessment".
Mr Barroso appealed: "Let us work together in a constructive and objective fashion. Let's not use stereotypes and myths. Let's have a rational and reasonable debate. Let us not give in to scaremongering and obfuscation."
The free movement of European citizens is a fundamental principle which must be upheld, the President of the European Commission has warned David Cameron.
Jose Manuel Barroso said he underlined this belief to David Cameron during a telephone conversation last night where Mr Cameron informed him of his plans for a crackdown on new EU arrivals claiming benefits.
Responding to a question from ITV News Europe Correspondent Emma Murphy, Mr Barroso said free movement was "one of the most valued" principles by European Union citizens.
The 10-minute call between the pair was described as "very cordial".
David Cameron has said Jose Manuel Barroso should start "respecting" the Conservative Party instead of "trying to lecture them" in response to Mr Barroso's claims that the Tories were heading for defeat at the hands of Ukip in next year's European elections.
The European Commission President yesterday warned that the increasingly anti-European stance adopted by the Conservative party could help Nigel Farage's Ukip beat them in the May 2014 polls.
"The Barroso thing did annoy me because, frankly, his job is to serve the members of the European Union and the British Conservatives in the European Parliament are an important party," Mr Cameron said on LBC.
"They are the governing party of the United Kingdom and he should be respecting their views rather than trying to lecture them. They've got an important contribution to make and I think he got it wrong, and I'll tell him that."
The Prime Minister revealed that the pair have had "some pretty robust exchanges" in the past, and suspected the conversation about Mr Barroso's comment would be one of them.
Continued hostility to the European Union could put years of peace in Europe at risk, according to the President of the European Commission.
In his State of the Union speech, Jose Manuel Barroso hailed European integration as "the valid answer" to the wars of the 20th century:
"Let me say this to all those who rejoice in Europe's difficulties and who want to roll back our integration and go back to isolation: the pre-integrated Europe of the divisions, the war, the trenches, is not what people desire and deserve.
"The European continent has never in its history known such a long period of peace as since the creation of the European Community. It is our duty to preserve it and deepen it."
Mr Barrasso also warned that the increasingly anti-European stance adopted by the Conservative party could help Nigel Farage's Ukip beat them in the May 2014 polls as voters will have to choose between "the original and the copy" and "prefer the original".