Detectives have ruled out the key sighting that underpinned the original Madeleine McCann investigation and adopted a new focus in the hunt.
On the day she disappeared Madeleine McCann asked her parents a question: “Why didn’t you come when Sean and I cried last night?”
Latest appeal is the parents' best - and most likely their last - hope to find out through police work what happened to their daughter.
I have just spoken to David Davis, an MP who is a prominent supporter of Andrew Mitchell, who says that they welcome this development because they want to challenge Pc Rowland to repeat those assertions under oath in court.
– police federation statement
The Federation has supported Pc Rowland with the provision of legal advicein respect of this matter. All subscribed members of the Police Federation areentitled to such support for legal representation as they see fit.
Andrew Mitchell said last week that he hoped Pc Toby Rowland would give evidence on oath as part of separate libel proceedings against the Sun newspaper.
Mr Mitchell told Channel 4 News: "What I do know is that I was stitched up; I was fitted up in Downing Street by armed police officers ...
"We believe that the CCTV shows that the account of Pc Toby Rowland is at very best inaccurate."
The police officer at the centre of the 'Plebgate' affair has filed a defamation case against former Tory Whip Andrew Mitchell, the Metropolitan Police Federation has confirmed.
Pc Toby Rowland, who has made the claim, was the officer on the Downing Street gate when Mr Mitchell was forced to push his bike through the pedestrian exit.
Mr Rowland's account of the altercation that followed differs from that of Mr Mitchell, but the Crown Prosecution Service decided there was not enough evidence to show which one was correct.
A Metropolitan Police bike safety initiative launched in the wake of a spike in the number of cyclist deaths in London will be "intensive and far-reaching"
– Superintendent Rob Revill, Safer Transport Command
Every road death is a needless tragedy that wreaks devastation on the victim's friends and family. Every serious injury is life-changing and distressing.
This operation will be intensive and far-reaching. Our aim is to reduce the appalling number of people who die or are injured on London's roads each year.
Traffic and Safer Transport officers will be out in force, and even officers who don't specialise in traffic policing will be watching and dealing accordingly with anyone they see breaking the law.
A surge in cycling deaths in London has prompted the Metropolitan Police to deploy 650 officers to the capital's busiest junctions later today.
The surge wiill see 2,500 officers will hand out leaflets and fixed penalty notices at 166 junctions marked as the capital's worst after six cyclists died in two weeks in collisions with lorries, buses, and coaches - some mere hours apart.
The man in charge of the Portuguese police investigation into Madeleine McCann's disappearance has said that Scotland Yard is at the same stage he was at in 2007.
Gonçalo Amaral, who was removed from the case after accusing the McCanns of involvement in their daughter;s disappearance, told ITV News that the latest e-fits are promising.
"Every one of us on the investigation at the time found that lead important – as we see now that it is," he said.
ITV News' Europe Correspondent Emma Murphy reports from Praia Du Luz in Portugal:
Gerry and Kate McCann have said they are "absolutely delighted with the overwhelming public response" to a BBC Crimewatch appeal, which was broadcast last night.
“We know that the public desperately want to help the search for Madeleine," they said in a statement.
"We are genuinely hopeful that one or more of these responses will lead to a major breakthrough in the investigation.
They added: "If anyone was in Praia da Luz around the time of Madeleine’s abduction and has not spoken to the Metropolitan Police, or if they know who any of the e-fits might be, please have the courage to come forward and speak to the police in confidence.”
German television station ZDF will stage a reconstruction of the events of the night of May 3, 2007 when Madeleine McCann disappeared from the room in which she was sleeping in Prais de Luz.
The fresh appeal follow's last night's Crimewatch appeal for new witnesses to come forward.
A special edition of the crime programme Aktenzeichen XY - Ungeloest which is translated as "File XY - Unsolved", will be aired on Wednesday night and will feature an appeal for information from Kate and Gerry McCann.
Police investigating the disappearance of Madeleine McCann have received nearly 1,000 calls and emails in response to a fresh appeal for information.
There have been more than 730 calls and 212 emails as a result of "specific lines of inquiry" about the events leading up to and surrounding Madeleine's abduction in Portugal on May 3 2007, Scotland Yard said.
Senior investigating officer Detective Chief Inspector Andy Redwood said detectives are working through the calls and material generated by Monday night's BBC Crimewatch programme.