Middlesbrough forward Cristhian Stuani has been charged for an "alleged act of violent conduct" by the Football Association.
An FA panel reviewed video footage of an incident during Saturday's 1-0 win over Preston which was missed by the match officials.
Middlesbrough have already confirmed they will appeal against the charge by the deadline of 6pm on Tuesday.
An FA statement read:
Cristian Stuani has been charged for an alleged act of violent conduct which was not seen by the match officials but caught on video. The Middlesbrough forward was involved in an incident in the 54th minute of their game with Preston North End on Saturday April 9, 2016.
The matter was referred to by a panel of three former elite referees who each reviewed the video footage independently of one another to determine whether they considered it a sending-off offence. For an FA charge to follow, the decision by the panel must be unanimous.
Middlesbrough head coach Aitor Karanka has resumed his duties at the Championship club.
There had been much speculation that the Spaniard would leave the Riverside after a failed attempt to smooth over a bust-up ahead of Sunday's 2-0 defeat at Charlton, which he missed.
But, in a statement on the club website on Monday evening, Boro said: "Middlesbrough Football Club can confirm that head coach Aitor Karanka will be resuming first-team duties with the club with immediate effect.
"We have 10 games left this season and as a club we are fully focussed on what remains of our Championship campaign."
Blackburn prevented Middlesbrough from going top of the Championship as a pair of Boro old boys outshone Jordan Rhodes on his Ewood Park return.
Both Burnley boss Sean Dyche and Hull manager Steve Bruce were present to see the team that could have moved above them in the table stumble to a 2-1 loss against Paul Lambert's side.
Hope Akpan's header early in the second half was added to by a goal from ex-Boro striker Danny Graham while goalkeeper Jason Steele, who came through the ranks at the Riverside, played a starring role in denying Rhodes on multiple occasions and only shipped one goal late on to Adam Forshaw.
Swansea loanee Matt Grimes' brilliant cross from the left was met by Akpan's head six yards out and he directed the ball back across Konstantopoulos for the opening goal.
Graham doubled the hosts' advantage with seven minutes to go by reaching the diminutive Jackson's flick-on from Steele's clearance, with both Dyche and Bruce having already left the ground.
Forshaw drove in a reply in stoppage time but Boro were unable to find a way past their old academy graduate Steele again.
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TEAM NEWS: Dimi, Kalas, Ayala, Gibson, Friend, Adomah, Clayton, Leadbitter, Downing, Fabbrini, Kike. http://t.co/HojrAc3Nxi
Subs: Mejias, Stephens, Stuani, Reach, Nsue, Forshaw, Nugent.
Middlesbrough have signed Leicester striker David Nugent for an undisclosed fee.
The one-cap England international has signed a three-year contract and goes straight into the Boro squad for Saturday's Sky Bet Championship home match against Bolton.
Boss Aitor Karanka told his club's official website: "I am delighted that David has agreed to join us because everyone knows what a good player he is.
"We have worked really hard to bring David to the club and everyone can see that we have not had to spend a crazy amount of money to bring him here. It's important to get the right players at the right price.
"The good thing is that David wants to join us and believes in our project and this club. I believe we are signing a very good player."
Middlesbrough are close to bringing former England midfielder Stewart Downing back to the Riverside Stadium from West Ham.
Press Association Sport understands that no fee has yet been agreed between the clubs, but that discussions have progressed and there is an appetite to do a deal, meaning the 30-year-old could be on his way to Teesside within days.
Downing has been left out of the Hammers squad for their Europa League clash with Birkirkara on Thursday evening.
Boro have been in talks with the Hammers for some time over their former Academy graduate, who left the club for Aston Villa in a £12million switch in July 2009.
Espanyol expect Christian Stuani to leave the Spanish club this summer with reports linking the Uruguay forward with a move to Middlesbrough.
Stuani joined Espanyol on a permanent transfer from Italian side Reggina in the summer of 2013 and is under contract with the Catalan outfit for another three seasons.
Reports in Spain suggest Stuani is set to join Boro on a £2.8million transfer fee, with half of the cash set to go to Reggina.
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