Dutch Prime Minister Mark Rutte has said two Turkish ministers were prevented from travelling in the Netherlands and campaigning among Turkish voters because Ankara had threatened sanctions against his government.
"We can never do business under this kind of blackmail," he said, adding: "We drew a red line."
Mr Rutte also said he was shocked to see Turkey's family affairs minister attempt to get to a Rotterdam rally by car after the Dutch government had made clear she was not welcome.
Fatma Betual Sayan was escorted back to the German border after a long standoff outside the Turkish consulate in Rotterdam.
Earlier, the Dutch government had withdrawn the landing rights of the plane carrying Turkey's foreign minister, who was also scheduled to attend a rally.
The ministers planned to urge Turkish expatriates to back a referendum, which would expand Turkey's president's powers, which will be held in April.
Protesters have been demonstrating outside the Dutch consulate in the Turkish city of Istanbul amid an escalating diplomatic row between Turkey and the Netherlands.
It comes after Turkey's president branded the Dutch "Nazi remnants" and "fascists" after his foreign minister's plane was denied landing permission on Saturday amid tensions over a banned Turkish rally.
In response, Dutch Prime Minister Mark Rutte said he understood Turkey's anger, but Mr Erdogan's remarks "way out of line".
Turkey's Family Affairs Minister was later blocked by Dutch police from entering the Turkish consulate in Rotterdam.
Prime Minister Binali Yildirim said Turkey will retaliate "in the harshest ways" to the Dutch authorities actions.
Turkey has said it will retaliate in the "harshest ways" to the Dutch government after the Netherlands barred Turkey's foreign minister from entering the country.
Prime Minister Binali Yildirim threatened on Sunday: "This situation has been protested in the strongest manner by our side, and it has been conveyed to Dutch authorities that there will be retaliation in the harshest ways... We will respond in kind to this unacceptable behaviour."
Dutch riot police broke up a protest by hundreds of backers of Turkish President Tayyip Erdogan demonstrating at the Turkish consulate in Rotterdam.
Police dressed in riot gear and on horseback attempted to disperse the crowd after a series of diplomatic incidents between Turkey and the Netherlands.
Demonstrators had gathered to show support for the Turkish government after the foreign minister and family affairs minister were prevented from campaigning for the referendum next month.
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