David Cameron has said tube drivers should only ever strike as a last resort.
Speaking during Prime Minister's Question, he said they were "well paid" so should not participate in such action.
It has been reported that tube drivers can earn up to £60,000, which is more than some doctors.
The Prime Minister has signalled that Britain could leave the European Convention on Human Rights after he insisted he ruled nothing out in achieving the Government's aims.
Mr Cameron told MPs gathered in the Commons for Prime Minister's Questions he wanted British judges making decisions in British courts, with the UK Parliament accountable to the people of the country.
He said: "We're very clear in what we want, which is British judges making decisions in British courts and also the British Parliament being accountable to the British people.
"Our plans set out in our manifesto do not involve us leaving the European Convention on Human Rights.
"But let's be absolutely clear: if we can't achieve what we need I rule out absolutely nothing in getting that done."
The Prime Minister has used a question from a Conservative Party backbencher to insist that the NHS will remain free at the point of use under his government.
During his first Prime Minister's Questions as leader of a majority Tory government, Mr Cameron also said that the health service will get an extra £8bn in extra annual funding.
David Cameron has faced the House of Commons for his first Prime Minister's Questions of the new Conservative government.Read the full story ›
Prime Minister David Cameron today ruled out a rise in VAT in the next Parliament if the Conservatives are in power after the general election.
Prime Minister David Cameron has begun the last PMQs before the general election by paying tribute to those killed in the plane crash in the French Alps.
Mr Cameron said: "I know the whole House will wish to join me in offering our deepest condolences to the families and friends of all those killed in yesterday's airbus crash in France.
"It is heartbreaking to hear about the schoolchildren, the babies, the families whose lives have been brought to an end."
Three British Nationals have been confirmed as being among the victims.
Mr Cameron said the the Foreign Office is "working urgently" to establish whether any further British nationals were among those on board the Germanwings flight.
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Prime Minister David Cameron and Labour leader Ed Miliband will go head-to-head in the House of Commons today for what is likely to be the final time.
Today's Prime Minister's Questions will be the last session before Parliament is dissolved on Monday ahead of the general election - and with both party leaders facing possible leadership questions if they fail to secure majorities, it could be the last time the pair face one another.
ITV News deputy political editor Chris Ship tweeted:
Last #pmqs involving both Cameron and Miliband today. Whatever happens in 6 weeks time, one of them won't be thier party leader any more
In May one presumes either Miliband will do a Hague (2001-style) or Cameron will do a Brown (2010-style). So enjoy the last Dave v Ed #pmqs
It comes just days after Mr Cameron told children's newspaper First News that PMQs was his least favourite part of leading the country.
I don't really enjoy Prime Minister's Questions. It's not a very good example to schoolchildren around the country because it's not a great reflection of what parliament does.
David Cameron and Ed Miliband will take part in the last Prime Minister's Question Time session before the General Election today.
As an ITV News poll reveals that Labour and the Conservatives are currently neck and neck among voters, the two leaders will have their final face off before ballots are cast on May 7.
The crucial final PMQs will see Miliband given six questions to challenge the Prime Minister on any chosen area of public policy.