Scotland were left facing their fourth wooden spoon in 11 years after a late collapse handed Italy a 22-19 RBS 6 Nations win at Murrayfield.
Mark Bennett's intercept try and 11 points from skipper Greig Laidlaw's boot looked to have put the hosts in command early on.
But scores from Joshua Furno and Giovanbattista Venditti, plus five points from kicker Kelly Haimona, allowed the Italians back into the contest just before the break.
Laidlaw added another penalty after the restart, but his side could not hold off a late surge from the Azzurri and left referee George Clancy no option but to hand the Italians a last-gasp penalty try, converted by Tommy Allan.
Ireland number eight Jordi Murphy will replace the injured Jamie Heaslip against England on Sunday in the only change to the champions side ahead of a potential Six Nations decider.
Heaslip cracked three vertebrae in Ireland's 18-11 victory over France two weeks ago in an incident that earned French lock Pascal Pape a 10-week ban, and Ireland hope he will be fit for their next game against Wales in Cardiff on March 14.
Exeter wing Jack Nowell will make his first England appearance for a year after being named on Friday to replace Jonny May in the team to face Ireland in what is shaping up to be a potential Six Nations decider Dublin on Sunday.
The slippery 21-year-old played in every match of last season's tournament, making his international debut in the opener against France, but has not been involved since after suffering bad knee and shoulder injuries.
However, his security under the high ball allied to some below-par defending by May in England's opening wins over Wales and Italy persuaded coach Stuart Lancaster to make the switch in one of two changes to the team that started both previous games.
The other change is an enforced one as Alex Goode starts at fullback in place of Mike Brown, who was ruled out after still feeling the effects of being knocked out at Twickenham two weeks ago.
England and Ireland have both won their opening two games and the victor on Sunday will be hot favourites to go on to take the title.
- 15-Alex Goode, 14-Anthony Watson, 13-Jonathan Joseph, 12-Luther Burrell, 11-Jack Nowell, 10-George Ford, 9-Ben Youngs; 8-Billy Vunipola, 7-Chris Robshaw (captain), 6-James Haskell, 5-George Kruis, 4-Dave Attwood, 3-Dan Cole, 2-Dylan Hartley, 1-Joe Marler
- Replacements: 16-Tom Youngs, 17-Mako Vunipola, 18-Henry Thomas, 19-Nick Easter, 20-Tom Croft, 21-Richard Wigglesworth, 22-Danny Cipriani, 23-Billy Twelvetrees
England full-back Mike Brown has been ruled out of Sunday's Six Nations match against Ireland after suffering a setback in his recovery from concussion, the Rugby Football Union has announced.
England head coach Stuart Lancaster said: "Mike and our medical staff have been diligent during every step of the graduated return to play protocol and they will continue to work to get him back to full health."
England retain hope that Mike Brown will participate in Sunday's Six Nations showdown with Ireland after completing the latest stage in the return to play protocols for concussion.
Brown was knocked unconscious following an accidental collision with Andrea Masi in the 47-17 victory over Italy and is a doubt for the potential title decider at the Aviva Stadium.
The reigning player of the Championship suffered a recurrence of symptoms last week and was forced to restart the protocols, but completed Tuesday's running session and will progress on to the next stage.
Alex Goode is on standby to deputise and Chris Pennell is also in the squad as full-back cover if Brown fails to prove his fitness by the deadline on Friday morning when the starting XV is announced.
Wing George North and prop Samson Lee have both been recalled by Wales for Saturday's Six Nations clash against France in Paris.
North has not played since taking two blows to his head during a 21-16 home defeat against England earlier this month, with Wales deciding to rest him for the Scotland game nine days ago.
But Northampton star North now returns, replacing his fellow 2013 British and Irish Lion Alex Cuthbert, while Lee - who was concussed in the England match - takes over from Aaron Jarvis. Two other changes following the victory over Scotland see starts for hooker Scott Baldwin and lock Luke Charteris.
Italy prop Martin Castrogiovanni is set to miss Italy's RBS 6 Nations trip to Scotland after being bitten in the face by a dog.
The 33-year-old Toulon player will be examined by medical staff to determine whether the wound beneath his nose and above his top lip will rule him out Saturday's game at Murrayfield, Edinburgh.
Castrogiovanni lives with three dogs, Nano the dachshund, Fatty the bulldog and Ettore the Bernese Mountain Dog, but it is understood a friend's pet administered a bite which needed 14 stitches.
The Italian Rugby Federation said the injury was the result of a "minor household incident" while announcing it had called up Wasps' Lorenzo Cittadini as cover.
If Castrogiovanni, who has been capped 109 times, cannot participate he will join Andrea Masi (severe cut) and Marco Bortolami (abductor muscle) on the sidelines.
The Azzurri are bottom of the Six Nations table after losing their two opening games to Ireland in Rome and then to England at Twickenham.
The Welsh revived their 6 Nations title hopes with a thrilling 26-23 win over Scotland at MurrayfieldRead the full story ›
The France lock was shown the yellow card for kneeing Jamie Heaslip in the back during Ireland's hard-fought 6 Nations win in DublinRead the full story ›
England boss Stuart Lancaster felt his team's victory over Wales last week had had a knock-on effect on the players in the 47-17 defeat of Italy.
Asked afterwards if his side had been suffering something of an emotional hangover at first from last Friday's 21-16 win in Wales, Lancaster told the BBC: "It looked that way, without a doubt.
"It was a soft try we conceded first up, and it took until half-time.
"I gave them a bit of a rev up and said 'listen boys, we need to up the intensity', and I thought we did that a little bit in the second half."
England lost Mike Brown to injury early on, and Lancaster added: "I don't think we can use that as a reason.
"I just think we were a little bit off, off the back of last week. We need to be better than that."