Britain's Kyle Edmund suffered a straight-sets defeat to Denis Shapovalov in the second round of the Western & Southern Open in Cincinnati.
Edmund, Britain's last hope of the tournament, was defeated 6-4 7-5 by his 19-year-old Canadian opponent in one hour, 53 minutes.
The 23-year-old held off five break points in the first set but failed to take advantage of his own break opportunities and eventually lost his serve in the final game.
Edmund failed to recover in the second set, falling below par on his first service game and handing his opponent an early 2-0 lead.
The Brit fought back and managed to level the score at 4-4, saving a match point at 5-5.
But Shapovalov dominated with a number of impressive backhands and despite needing four match points, ultimately broke Edmund's serve to advance to the third round on his tournament debut.
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World number one Rafael Nadal has pulled out of this week's Cincinnati Masters.
The 32-year-old Spaniard, who won the title in 2013, feels he needs a rest following Sunday's victory at the Rogers Cup in Toronto.
"I am very sorry to announce that I won't be playing in Cincinnati this year," Nadal wrote on Twitter.
"No other reason than personally taking care of my body and trying to keep as healthy as I feel now.
"I am very thankful to my friend Andre Silva, tournament director of the Cincinnati tournament, who after speaking to him on the phone understood what I said and understands the situation.
"I am sure the tournament will be a success and I wish him and his team all the best. I am also sad for those amazing fans in Cincy and around who always gave me great support.
"I am sure I'll see them next year and I am sorry I won't be with them this year. Thanks for all the support as always."
Nadal, the 17-time grand slam winner, collected his 80th career title on Sunday by beating Greek youngster Stefanos Tsitsipas 6-2 7-6 (7/4).
Kyle Edmund failed to clear the opening round of the Rogers Cup in Toronto after suffering a bruising 6-1 6-2 loss to Diego Schwartzman.
After rain delayed play, the British number one was dismissed in an hour by the Argentine number 11 seed on Monday evening.
Edmund appeared outgunned by Schwartzman from the off, losing his first service game as the world number 12 notched up three.
The Briton, ranked 16th by the ATP, made it on to the scoreboard in the fourth game, but he was broken again in the sixth and went on to lose the set.
Edmund, who after a bye was knocked out of the Citi Open's second round by Sir Andy Murray a week ago, appeared to have regained some composure as play resumed.
However, with the score 2-2, the match ran away from him in the fifth game, Schwartzman breaking his serve.
Edmund managed to save one break point in the seventh, but he was overcome by his opponent who took the match in the eighth.
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