Ospreys wing Eli Walker has been released from Wales' Rugby World Cup squad due to a hamstring injury.
The release is a fresh blow to the squad as once-capped Walker, 25, was called in to replace Leigh Halfpenny after a knee injury.
Due to the nature of the impending competition and the potential for his injury to extend into the latter stages of the Pool stage, the decision has been made to release Eli at present.
Gloucester flanker Ross Moriarty, whose father Paul and uncle Richard both played in the 1987 World Cup, has been called into the squad.
The WRU said twice-capped Gloucester back-rower Moriarty, 21, will link up with Warren Gatland's men tomorrow.
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Wales head coach Warren Gatlin has urged his squad not to feel sorry for themselves after scrum-half Rhys Webb became the second player to be ruled out of the World Cup through injury.
Webb was injured in Saturday's final warm-up game with Italy in Cardiff and joins Lions full-back Leigh Halfpenny on the sidelines.
Despite the injuries, Gatlin insists he is happy with his squad and that his players will not feel sorry for themselves going into the tournament:
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Wales scrum-half Rhys Webb has been ruled out of the World Cup with a foot injury, the Welsh Rugby Union has announced.
Webb, last season's Welsh player of the year, was injured during Wales' 23-19 World Cup warm-up victory over Italy in Cardiff on Saturday.
"Scrum-half Rhys Webb has been ruled out of @rugbyworldcup with foot injury. Further examination on foot and knee to come," the WRU said on its official Twitter account.
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Wales full back Leigh Halfpenny has been ruled out of the Rugby World Cup after suffering a cruciate ligament injury.
The 26-year-old was taken off on a stretcher during Wales' final warm-up game against Italy on Saturday.
A spokesman for the Welsh squad said: "Leigh will undergo further examination and assessment before further details are announced."
Chris Coleman promises his Wales side will not be distracted by the hype as they enter uncharted territory.
Victory over Israel in Cardiff on Sunday evening will stamp Wales' ticket to next summer's Euro 2016 finals and end a 58-year wait for the nation to play at a major championship.
"We all know what the possibilities are and what it's going to be like," Coleman said at his pre-match press conference.
"There's going to be a fantastic atmosphere but we mustn't get caught up in it because if you do that you stop thinking about what got you there in the first place.
"We'll stick to the game plan and I'm not bothered about the performance being remembered for loads of goals and lots of excitement.
"If that's the case and we came out on top, great, but we'll stick to what we've been working on and stay calm and focused."