Dustin Brown, who knocked Rafael Nadal out of Wimbledon, has crashed out of the tournament himself.
The German tennis player could not repeat his success as he was defeated by Viktor Troicki of Serbia in the third round.
Britain's chances of a Wimbledon win will be on a knife edge later as Andy Murray seeks a place in the last 16.
Murray, who won the men's singles title in 2013 and is the bookies second favourite to claim it again, is up against Italy's Andreas Seppi in the third round.
He and world number 111 James Ward - who faces Canadian Vasek Pospisil today - are the only Britons remaining after Heather Watson was knocked out yesterday.
British number one Watson came within two points of eliminating 20-time grand slam winner Serena Williams with the backing of a passionate local crowd.
But she was left heartbroken when the American fought back to set up a Williams family tie with her older sister Venus in the next round.
British number one Heather Watson has been knocked out of Wimbledon by Serena Williams, despite a battling performance which almost saw the underdog take a thrilling final set.
Watson lost 6-2, 4-6, 7-5 after a remarkable comeback and led in the final set 3-0 before being pegged back. Speaking following the game, Williams - the tournament's top seed - praised her opponent and said Watson "should have won".
"She really gave her all and showed how good she is," she told BBC Sport.
British number one Heather Watson has taken Serena Williams in the third set of their third-round clash at Wimbledon this evening.
Watson, making only her second-ever appearance in Round 3, broke Williams' serve twice, to win the set 6-4, after losing the first by six games to two.
Top seed Novak Djokovic of Serbia has won 6-3 6-3 6-3 against Australia's 27th seed Bernard Tomic in the third round at Wimbledon
Russian fourth seed Maria Sharapova has won 6-4 6-3 against Romania's 29th seed Irina-Camelia Begu in the third round at Wimbledon.
Spectators and staff at Wimbledon have paid tribute to the 38 people killed in last week's terror attack in Tunisia.
Hundreds stood in silence on Murray Mound at midday before clapping once the minute was over.
Matches started at 12.15pm rather than the usual 11.30am to allow players, staff and fans to take part.
Dustin Brown recorded a famous win over Rafael Nadal at Wimbledon - here's 10 things you may not have known about himRead the full story ›
British number one Heather Watson will meet World number one and current Wimbledon champion Serena Williams in the third round today.Read the full story ›
Two-time Wimbledon champion Rafa Nadal has been knocked out in the second round by Germany's Dustin Brown.